酵素研究チーム

Accomplishments from Enzyme Research Team

Publications
[90] Takeshi Yoshizumi, Miwa Yamada, Mieko Higuchi-Takeuchi, Ken’ichiro Matsumoto, Seiichi Taguchi, Minami Matsui, Keiji Numata*, Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants. Plant Biotechnology, in press.
[89] Jo-Ann Chuah, Akimasa Matsugami, Fumiaki Hayashi, Keiji Numata*, Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights. Biomacromolecules, Volume 17(11), pp3547–3557, 2016.
American Chemical Society, Chemical & Engineering News: A step toward mitochondrial gene therapy.
[88] Keiji Numata*, Yoko Horii, Yoko Motoda, Tokumi Hirai, Chikako Nishitani, Satoru Watanabe, Takanori Kigawa, Yutaka Kodama, Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance. Plant Biotechnology, in press.
[87] Yoke-Ming Wong, Hiroyasu Masunaga, Jo-Ann Chuah, Kumar Sudesh, Keiji Numata*, Enzyme-mimic peptide assembly to achieve amidolytic activity. Biomacromolecules,Volume 17(10), pp3375–3385, 2016.
[86] Mieko Higuchi-Takeuchi, Kumiko Morisaki, Keiji Numata*. A screening method for the isolation of polyhydroxyalkanoate-producing purple non-sulfur photosynthetic bacteria from natural seawater. Frontiers in Microbiology, Special issue “Biotechnology of microalgae, based on molecular biology and biochemistry of eukaryotic algae and cyanobacteria”,7:1509, 2016.
[85] Chayatip Insomphun, Jo-Ann Chuah, Shingo Kobayashi, Tetsuya Fujiki, Keiji Numata*, Influence of hydroxyl groups on the cell viability of polyhydroxyalkanoate scaffolds for tissue engineering. ACS Biomaterials Science and Engineering, in press.
[84] Pei-Shze Mok, Diana Hooi-Ean Ch’ng, Soo-Peng Ong, Keiji Numata, Kumar Sudesh*, Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film. AMB Express, 6:97, 2016.
[83] Mieko Higuchi-Takeuchi, Kumiko Morisaki, Kiminori Toyooka, Keiji Numata*, Synthesis of high-molecular-weight polyhydroxyalkanoates by marine photosynthetic purple bacteria. PLOS ONE, 11(8): e0160981, 2016.
[82] Jo-Ann Chuah, Yoko Horii, Keiji Numata*, Peptide-Derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants. Journal of Visualized Experiments (JoVE), in press.
[81] Ali D. Malay, Hiroe Watanabe, Kenjiro Yazawa, Ryota Sato, Nao Ifuku, Hiroyasu Masunaga, Takaaki Hikima, Juan Guan, Biman B. Mandal, Siriporn Damrongsakkul, Keiji Numata*. Relationships between physical properties and sequences in silkworm silks. Scientific Reports. 6:27573, 2016.
[80] Kenjiro Yazawa, Keiji Numata*. Papain-catalyzed synthesis of peptides containing a nylon unit. Polymers, Special Issue "Enzymatic Polymer Synthesis", Volume 8(5), 194, 2016.
[79] Kiaw Kiaw Ng, Yoko Motoda, Satoru Watanabe, Takanori Kigawa, Ahmad Sofiman Othman, Yutaka Kodama, Keiji Numata* Intracellular delivery of proteins in intact plant via fusion peptides. PLoS ONE. 11(4), e0154081, 2016.
[78] Kenjiro Yazawa, Michihiro Sugahara, Katsuhide Yutani, Michiyo Takehira, and Keiji Numata*. Derivatization of Proteinase K with Heavy Atoms Enhances Its Thermal Stability. ACS Catalysis. Volume 6, pp3036–3046, 2016.
[77] Sachiko Nitta*, Ayaka Komastu, Taisei Ishii, Hiroyuki Iwamoto, Keiji Numata*, Synthesis of peptides with narrow molecular weight distributions via exopeptidase-catalyzed aminolysis of hydrophobic amino acid alkyl esters. Polymer Journal. Volume 48, pp955-961, 2016.
[76] Michihiro Sugahara*, Changyong Song, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Takanori Nakane, Fumiaki Yumoto, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, So Iwata. Oil-free hyaluronic acid matrix for serial femtosecond crystallography. Scientific Reports. 6, pp24484, 2016.
[75] Chayatip Insomphun, Shingo Kobayashi, Tetsuya Fujiki, Keiji Numata*. Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli. AMB Express, 6 (1), pp1-8 2016.
[74] Yinan Ma, Zhibo Li*, Keiji Numata*, Synthetic short peptides for rapid fabrication of monolayer cell sheets. ACS Biomaterials Science and Engineering. Volume 2 (4), pp697-706, 2016.
[73] Kenjiro Yazawa, Kana Ishida, Hiroyasu Masunaga, Takaaki Hikima, Keiji Numata*, Influence of water content on the β-sheet formation, thermal stability, water removal, and mechanical properties of silk materials. Biomacromolecules. Volume 17 (3), pp1057-1066, 2016.
[72] Kousuke Tsuchiya*, Keiji Numata*, Papain-Catalyzed Chemoenzymatic Synthesis of Telechelic Polypeptides Using Bis(leucine ethyl ester) Initiator. Macromolecular Bioscience. Volume 16, Issue 7, pp1001–1008, 2016 (Front Cover).
[71] Leo Simmons, Kousuke Tsuchiya*, Keiji Numata*. Chemoenzymatic modification of silk fibroin with poly(2,6-dimethyl-1,5-phenylene ether) using horseradish peroxidase. RSC Advances. Volume 6, pp28737-28744, 2016.
[70] Jose Manuel Ageitos, Kenjiro Yazawa, Ayaka Tateishi, Kosuke Tsuchiya, Keiji Numata*, Benzyl ester group of amino acid monomers broadens the substrate specificity and enhance the affinity of papain in chemo-enzymatic copolymerization. Biomacromolecules, Volume 17, pp314–323, 2016.
[69] Yinan Ma, Ryota Sato, Zhibo Li*, Keiji Numata*, Chemoenzymatic synthesis of oligo(L-cysteine) for use as thermal stable bio-based material. Macromolecular Bioscience, Volume 16, pp151–159, 2016.
[68] Jose Manuel Ageitos, Jo-Ann Chuah, Keiji Numata* Design considerations for properties of nanocarriers on disposition and efficiency of drug and gene delivery. Nanomedicines: design, delivery and detection. Edited by Martin Braddock, Royal Society of Chemistry, (2016).
[67] Keiji Numata*, Ryota Sato, Kenjiro Yazawa, Takaaki Hikima, Hiroyasu Masunaga. Crystal structure and physical properties of Antheraea yamamai silk fibers: long poly(alanine) sequences are partially in crystalline region. Polymer, Volume 77, pp87-94, 2015.
[66] Keiji Numata*, Hiroyasu Masunaga, Takaaki Hikima, Sono Sasaki, Kazuhide Sekiyama, Masaki Takata. Use of extension-deformation-based crystallization of silk fibres to differentiate their functions in nature. Soft Matter, Volume 11, pp6335-6342, 2015.
[65] Alex C. Levine, Angelina Sparano, Frederick F. Twigg, Keiji Numata, Christopher T. Nomura*. Influence of cross-linking on the physical properties and cytotoxicity of polyhydroxyalkanoate (PHA) scaffolds for tissue engineering. ACS Biomaterials Science & Engineering, Volume 1, pp567–576, 2015.
[64] Keiji Numata*. Poly(amino acid)s/polypeptides as potential functional and structural materials (Invited Focus review). Polymer Journal, Volume 47 Issue 8, pp537-545, 2015.
[63] Jose Manuel Ageitos, Jo-Ann Chuah, Keiji Numata*, Chemo-Enzymatic Synthesis of Linear and Branched Cationic Peptides: Evaluation as Gene Carriers, Macromolecular Bioscience, Volume 15, Issue 7, pp990–1003, 2015.
[62] Keiji Numata*, Kumiko Morisaki. Screening of marine bacteria to synthesize polyhydroxyalkanoate from lignin: Contribution of lignin derivatives to biosynthesis by Oceanimonas doudoroffii. ACS Sustainable Chemistry and Engineering, Volume 3, Issue 4, pp569–573, 2015.
[61] Keiji Numata*, Yoko Motoda, Satoru Watanabe, Takashi Osanai, and Takanori Kigawa. Co-expression of two polyhydroxyalkanoate synthase subunits from Synechocystis sp. PCC 6803 by cell free synthesis and their specific activity for polymerization of 3-hydroxybutyryl-CoA. Biochemistry, Volume 54, Issue 6, pp1401–1407, 2015.
[60] Yoke-Ming Wong, Yu Hoshino, Kumar Sudesh, Yoshiko Miura, Keiji Numata*. Optimization of poly(N-isopropylacrylamide) as an artificial amidase. Biomacromolecules, Volume 16, Issue 1, pp411-421, 2015.
[59] Jo-Ann Chuah, Takeshi Yoshizumi, Yutaka Kodama, Keiji Numata*. Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers. Scientific Reports, 5, 7751 2015.
[58] Manoj Lakshmanan, Takeshi Yoshizumi, Kumar Sudesh, Yutaka Kodama, Keiji Numata*, Double stranded DNA introduction into intact plant using peptide-DNA complexes. Plant Biotechnology, Volume 32, Issue 1, pp39-45, 2015.
[57] Peter James Baker, Siddharth V Patwardhan, Keiji Numata*, Synthesis of Homopolypeptides by Aminolysis Mediated by Proteases Encapsulated in Silica Nanospheres. Macromolecular Bioscience,Volume 14, Issue 11, pp1619-26, 2014.
[56] Kenjiro Yazawa, Keiji Numata*. Recent advances in the chemoenzymatic peptide syntheses. Molecules, Special Issue "Peptide Chemistry", Volume 19, Issue 9, pp13755-13774, 2014.
[55] Keiji Numata*, Peter James Baker, Synthesis of adhesive peptides similar to those found in blue mussel (Mytilus edulis) using papain and tyrosinase. Biomacromolecules, Volume 15, Issue 8, pp3206-12, 2014.
[54] Satoshi Tomizawa, Jo-Ann Chuah, Keiji Matsumoto, Yoshiharu Doi, Keiji Numata* Understanding the limitations in the biosynthesis of polyhydroxyalkanoate (PHA) from lignin derivatives. ACS Sustainable Chemistry and Engineering, Volume 2, Issue 5, pp1106–1113, 2014.
[53] Keiji Numata*, Misato Ohtani, Takeshi Yoshizumi, Taku Demura, Yutaka Kodama. Local gene silencing in plants via synthetic dsRNA and carrier peptide. Plant Biotechnology Journal (Wiley), Volume 12, Issue 8, pp1027-1034, 2014.
[52] Kazunori Ushimaru, Yoko Motoda, Keiji Numata, Takeharu Tsuge*. Phasin Proteins Activate Aeromonas caviae Polyhydroxyalkanoate (PHA) Synthase but not Ralstonia eutropha PHA Synthase. Applied Environmental Microbiology, Volume 80, Issue 9, pp2867-2873, 2014.
[51] Takashi Osanai, Akira Oikawa, Keiji Numata, Ayuko Kuwahara, Hiroko Iijima, Yoshiharu Doi, Kazuki Saito, Masami Yokota Hirai*. Pathway-Level Acceleration of Glycogen Catabolism by Response Regulator Rre37 in the Cyanobacterium Synechocystis sp. PCC 6803. Plant Physiology, Volume 164, Volume 4, pp1831-1841, 2014.
[50] Jenny Fagerland, Anna Finne Wistrand, Keiji Numata*. Short one-pot chemo-enzymatic synthesis of L-lysine and L-alanine diblock cooligopeptides. Biomacromolecules, Volume 15, Issue 3, pp735-743, 2014.
[49] Keiji Numata*, Shoya Yamazaki, Jo-Ann Chuah, Takuya Katashima, Naofumi Naga, Takamasa Sakai. Silk-Pectin Hydrogel with Superior Mechanical Properties, Biodegradability, and Biocompatibility. Macromolecular Bioscience, Volume 14, Issue 6, pp799-806,2014 (Back Cover)
[48] Jo-Ann Chuah, David Kaplan, Keiji Numata*. Engineering Peptide-based Carriers for Drug and Gene Delivery. Engineering in Translational Medicine. Springer, London (2014).
[47] Chayatip Insomphun, Jun Mifune, Izumi Orita, Keiji Numata, Satoshi Nakamura, Toshiaki Fukui*. Modification of ß-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil. Journal of Bioscience and Bioengineering, Volume 117, Issue 2, pp184-190,2014.
[46] Satoshi Tomizawa, Chuah Jo-Ann, Misato Ohtani, Taku Demura and Keiji Numata. Biosynthesis of Polyhydroxyalkanoate by Marine Bacteria Vibrio sp. Using Sugars, Plant Oil and Unsaturated Fatty Acids as a Sole Carbone Source. Green Polymer Chemistry: Biocatalysis and Materials II, Chapter 15, pp 211–221, ACS Symposium Series, Vol. 1144, ISBN13: 9780841228955 eISBN: 9780841228962, 2014.
[45] Jose Manuel Ageitos, Peter James Baker, Michihiro Sugahara, Keiji Numata*. Proteinase K-catalyzed synthesis of linear and star oligo-L-phenylalanine conjugates. Biomacromolecules, Volume 14, Issue 10, pp3635-3642,2013.
[44] Takashi Osanai, Keiji Numata, Akira Oikawa, Kiminori Toyooka, Mayuko Sato, Ayuko Kuwahara, Hiroko Iijima, Yoshiharu Doi, Kan Tanaka, Kazuki Saito, and Masami Yokota Hirai*. Overexpression of an RNA Polymerase Sigma Factor SigE Increases Bioplastic Production in Cyanobacteria. DNA Research, Volume 20, Issue 6, pp525-535,2013.
[43] Koichiro Tachibana, Yuichi Urano, Keiji Numata*. Biodegradability of Nylon 4 Film in a Marine Environment. Polymer Degradation and Stability, Volume 98, Issue 9, pp1847–1851, 2013.
[42] Jo-Ann Chuah, Satoshi Tomizawa, Miwa Yamada, Takeharu Tsuge, Yoshiharu Doi, Kumar Sudesh*, Keiji Numata*. Characterization of Site-Specific Mutations in a Short-Chain-Length-Medium-Chain-Length Polyhydroxyalkanoate Synthase: in vivo and in vitro Studies on Enzymatic Activity and Substrate Specificity. Applied and Environmental Microbiology, Volume 79, Issue 12, pp3813-3821, 2013.
[41] Keiji Numata*, Kumiko Morisaki, Satoshi Tomizawa, Misato Ohtani, Taku Demura, Masayuki Miyazaki, Yuichi Nogi, Shigeru Deguchi and Yoshiharu Doi. Synthesis of Poly- and Oligo(hydroxyalkanoate)s by Deep-Sea Bacteria, Colwellia spp., Moritella spp. and Shewanella spp. Polymer Journal, Volume 45, pp1094-1100, 2013 (Selected as a Featured Article, October 2013).
[40] Miwa Yamada, Shuntaro Takahashi, Yoshio Okahata, Yoshiharu Doi, Keiji Numata*. Monitoring and Kinetic Analysis of the Molecular Interactions by which a Repressor Protein, PhaR, Binds to Target DNAs and Poly[(R)-3-hydroxybutyrate]. AMB express, Volume 3, 6, 2013.
[39] Sachiko Kaihara Nitta, Keiji Numata*. Biopolymer-based Nanoparticles for Drug/Gene Delivery and Tissue Engineering. International Journal of Molecular Sciences, Special Issue "Bioactive Nanoparticles 2012", Volume 14, Issue 1, pp1629-1654, 2013.
[38] Manoj Lakshmanan, Yutaka Kodama, Takeshi Yoshizumi, Kumar Sudesh, Keiji Numata*. Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers. Biomacromolecules, Volume 14, Issue 1, pp10-16, 2013.
[37] Keiji Numata*. "Silk Hydrogels for Tissue Engineering and Dual Drug Delivery." Silk Biomaterials in Tissue Engineering and Regenerative Medicine, Edited by S.C.Kundu, 2013.
[36] Jo-Ann Chuah, Miwa Yamada, Seiichi Taguchi, Sudesh Kumar, Yoshiharu Doi, Keiji Numata*. Biosynthesis and Characterization of Polyhydroxyalkanoate Containing 5-Hydroxyvalerate (5HV) Units: Effects of 5HV Units on Biodegradability, Cytotoxicity, Mechanical and Thermal Properties. Polymer Degradation and Stability, Volume 98, Issue 1, pp331-338, 2013.
[35] Keiji Numata*, Yoko Motoda, Satoru Watanabe, Takanori Kigawa and Yoshiharu Doi. Active Intermediates of Polyhydroxyalkanoate Synthase from Aeromonas caviae in Polymerization Reaction. Biomacromolecules, Volume13, Issue 11, pp3450–3455, 2012.
[34] Peter J Baker, Keiji Numata*. "Polymerization of Peptide Polymers for Biomaterial Applications" Polymer Science, ISBN 979-953-307-969-0. 2012.
[33] Keiji Numata*, Shoya Yamazaki, Naofumi Naga. Biocompatible and Biodegradable Dual-Drug Release System Based on Silk Hydrogel Containing Silk Nanoparticles. Biomacromolecules, Volume 13, Issue 5, pp1383-9, 2012 (Most read paper between April and June 2012).
[32] Peter James Baker, Keiji Numata*, Chemo-Enzymatic Synthesis of Poly(L-Alanine) in Aqueous Environment. Biomacromolecules, Volume 13, Issue 4, pp947-51, 2012.
[31] Keiji Numata*, Yoshiharu Doi. Biosynthesis of Polyhydroxyalkanoate by Novel Facultative Anaerobe Vibrio sp. under Marine Conditions. Marine Biotechnology, Volume 14, Issue 3, pp323-331, 2012.
[30] Keiji Numata*, Takuya Katashima, Takamasa Sakai. The State of Water, Molecular Structure and Cytotoxicity of Silk Hydrogels. Biomacromolecules, Volume 12, Issue 6, pp2137-44,2011.
[29] Keiji Numata*, Yoshiharu Doi. Dual Biosyntheses of Poly[(R)-3-hydroxybutyric acid] and Silk Protein to Fabricate Bio-functional Bioplastic. Polymer Journal, Volume 43, pp642-647, 2011 (Selected as a Featured Article, Top3 Downloaded Article in July 2011)

Previous works
[28] Keiji Numata, Aneta J Mieszawska, Laura A. Kvenvold, David L Kaplan*. Silk-Based Nano-Complexes with Tumor Homing Peptides for Tumor-Specific Gene Delivery. Macromolecular Bioscience Volume 12, Issue 1, pp75-82, 2012.
[27] Keiji Numata, Michaela R Reagan, Robert H Goldstein, Michael Rosenblatt, David L Kaplan*. Spider Silk-Based Gene Carriers for Tumor Cell-Specific Delivery. Bioconjugate Chemistry, Volume 22, Issue 8, pp1605-1610,2011.
[26] Keiji Numata, David L Kaplan*. "Chapter 20: Biologically-Derived Scaffolds", Advanced Wound Repair Therapies, Edited by David Farrar and Bryan Greener, pp524-551, Woodhead Publishing, 2011. Book DOI: 10.1533/9780857093301, ISBN: 978-1-84569-700-6 (Print) 978-0-85709-330-1 (Online), Chapter DOI: 10.1533/9780857093301.4.524. Online date: Monday, June 27, 2011.
[25] Vikas Yadav, Bruce Panilaitis, Hai Shi, Keiji Numata, Kyongbum Lee, David L Kaplan*. N-Acetylglucosamine 6-Phosphate Deacetylase (nagA) Is Required for N-acetyl Glucosamine Assimilation in Gluconacetobacter xylinus. PLoS ONE 6(6): e18099, 2011.
[24] Sílvia Gomes, Keiji Numata, Isabel B. Leonor, João F. Mano, Rui L. Reis, David L. Kaplan*. AFM Study of Morphology and Mechanical Properties of A Chimeric Spider Silk and Bone Sialoprotein Protein for Bone Regeneration, Biomacromolecules, Volume 12, Issue 5, pp1675-1685,2011.
[23] Keiji Numata, and David L. Kaplan*. Differences in Cytotoxicity of β-Sheet Peptides Originated from Silk and Amyloid β. Macromolecular Bioscience Volume 11, Issue 1, pp60-64,2011.
[22] Keiji Numata, David L Kaplan*. Silk-Based Delivery Systems of Bioactive Molecules. Advanced Drug Delivery Reviews Volume 62, Issue 15, pp1497-1508, 2010.
[21] Sejuti Sengupta, Sang-Hyug Park, Eun Seok Gil, Atur Patel, Keiji Numata, Chia-Li Lu, David L. Kaplan*. Quantifying Osteogenic Cell Degradation of Silk Biomaterials. Biomacromolecules Volume 11, Issue 12, pp3592-3599, 2010.
[20] Keiji Numata, David L Kaplan*. Silk-Based Gene Delivery Vehicles to Contain Membrane-Destabilizing Peptides: Control of Transfection by Their Secondary Structure. Biomacromolecules Volume 11, Issue 11, pp3189-3195, 2010.
[19] Keiji Numata, Juliana Hamasaki, Balajikarthick Subramanian, David L Kaplan*. Gene Delivery Mediated by Recombinant Silk Proteins Containing Cationic and Cell Binding Motifs. Journal of Controlled Release Volume 146, Issue 1, pp136-143, 2010.
[18] Nina Tandon, Anna Marsano, Robert Maidhof, Keiji Numata, Chrystina Montouri-Sorrentino, Christopher Cannizzaro, Joel Voldman, Gordana Vunjak-Novakovic*. Surface-Patterned Electrode Bioreactor for Electrical Stimulation. Lab on a Chip Volume 10, Issue 6, pp692-700, 2010.  
[17] Keiji Numata, David L Kaplan*. Mechanisms of Enzymatic Degradation of Amyloid Beta Microfibrils Generating Nanofilaments and Nanospheres Related to Cytotoxicity. Biochemistry Volume 49, Issue 15, pp3254-3260, 2010.
[16] Rangam Rajkhowa, Eun Seok Gil, Jonathan Kluge, Keiji Numata, Lijing Wang, Xungai Wang, David L Kaplan*. Reinforcing Silk Scaffolds with Silk Particles. Macromolecular Bioscience Volume 10, Issue 6, pp599-611, 2010.
[15] Keiji Numata, Peggy Cebe, David Kaplan*. Mechanism of Enzymatic Degradation of Beta Sheet Crystals. Biomaterials Volume31, Issue 10, pp2926-2933, 2010.
[14] Guokui Qin, Shaul Lapidot, Keiji Numata, Xiao Hu, Sigal Meirovitch, Mara Dekel, Itai Podoler, Oded Shoseyov, David L. Kaplan*. Expression, Cross-Linking and Characterization of Recombinant Chitin Binding Resilin. Biomacromolecules Volume 10, Issue 12, pp3227-3234, 2010.
[13] Keiji Numata, Balajikarthick Subramanian, Heather Currie, David Kaplan*. Bioengineered Silk Protein-Based Gene Delivery Systems. Biomaterials Volume 30, Issue 29, pp5775-5784, 2009.
[12] Keiji Numata, Hideki Abe, Tadahisa Iwata*. Biodegradability of Poly(hydroxyalkanoate) Materials. Materials Volume 2, Issue 3, pp1104-1126, 2009.
[11] Keiji Numata, Anna Finne-Wistrand, Ann-Christine Albertsson, Yoshiharu Doi, Hideki Abe*. Enzymatic Degradation of Monolayer for Poly(lactide) Revealed by Real-Time Atomic Force Microscopy: Effects of Stereochemistry, Molecular Weight and Molecular Branches on Hydrolysis Rate. Biomacromolecules Volume 9, Issue 8, pp2180-2185,2008.
[10] Keiji Numata*, Hideki Abe, Yoshiharu Doi. Enzymatic Processes for Biodegradation of Poly(hydroxyalkanoate)s Crystals. Canadian Journal of Chemistry (Special Issue: Dedicated to Professor R. Marchessault) Volume 86, Issue 6, pp471-483,2008.
[9] Keiji Numata, Rajiv K. Srivastava, Anna Finne-Wistrand, Ann-Christine Albertsson*, Yoshiharu Doi, Hideki Abe*. Branched Poly(lactide) Synthesized by Enzymatic Polymerization: Effects of Molecular Branches and Stereochemistry on Enzymatic Degradation and Alkaline Hydrolysis. Biomacromolecules Volume 8, Issue 10, pp3115-3125,2007.
[8] Keiji Numata, Koichi Yamashita, Masahiro Fujita, Takeharu Tsuge, Ken-ichi Kasuya, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*.Adsorption and Hydrolysis Reactions of Poly(hydroxybutyric acid) Depolymerases Secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto Poly[(R)-3-hydroxybutyric acid]. Biomacromolecules Volume 8, Issue 7, pp2276-2281,2007.
[7] Keiji Numata, Shun Sato, Masahiro Fujita, Takeharu Tsuge, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*.Adsorption Effects of Poly(hydroxybutyric acid) Depolymerase on Chain-Folding Surface of Polyester Single Crystals Revealed by Mutant Enzyme and Frictional Force Microscopy.  Polymer Degradation and Stability Volume 92, Issue 1, pp176-183,2007.
[6] Keiji Numata, Yoshihiro Kikkawa, Takeharu Tsuge, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*.Adsorption of Biopolyester Depolymerase on Silicon Wafer and Poly[(R)-3-hydroxybutyric acid] Single Crystal Revealed by Real-Time Atomic Force Microscopy.  Macromolecular Bioscience Volume 6, Issue 1, pp41-50,2006.
[5] Koichi Yamashita*, Miwa Yamada, Keiji Numata, Seiichi Taguchi.Nonspecific Hydrophobic Interactions of a Repressor Protein, PhaR, with Poly[(R)-3-hydroxybutyrate] Film Studied by Quartz Crystal Microbalance. Biomacromolecules Volume 7, Issue 8, pp2449-2454,2006.
[4] Keiji Numata, Yoshihiro Kikkawa, Takeharu Tsuge, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*. Enzymatic Degradation Processes of Poly[(R)-3-hydroxybutyric acid-co-(R)-3-hydroxyvaleric acid] Single Crystals Revealed by Atomic Force Microscopy: Effects of Molecular Weight and Second-Monomer Composition. Biomacromolecules Volume 6, Issue 4, pp2008-2016,2005.
[3] Takeharu Tsuge, Kohtaroh Yano, Shin-ichi Imazu, Keiji Numata, Yoshihiro Kikkawa, Hideki Abe, Seiichi Taguchi, Yoshiharu Doi*. Biosynthesis of Polyhydroxyalkanoate (PHA) Copolymers from Fructose Using Wild-Type and Laboratory-Evolved PHA Synthases. Macromolecular Bioscience Volume 5, Issue 2, pp112-117, 2005.
[2] Keiji Numata, Takuya Hirota, Yoshihiro Kikkawa, Takeharu Tsuge, Tadahisa Iwata, Hideki Abe, Yoshiharu Doi*. Enzymatic Degradation Processes of Lamellar Crystals in Thin Films for Poly[(R)-3-hydroxybutyric acid] and Its Copolymers Revealed by Real-Time Atomic Force Microscopy. Biomacromolecules Volume 5, Issue 6, pp2186-2194,2004.
[1] Yoshihiro Kikkawa, Takuya Hirota, Keiji Numata, Takeharu Tsuge, Hideki Abe, Tadahisa Iwata, Yoshiharu Doi*. In-situ Atomic Force Microscopy Observation of Enzymatic Degradation in Poly(hydroxyalkanoic acid) Thin Films: Normal and Constrained Conditions. Macromolecular Bioscience Volume 4, Issue 3, pp276-285, 2004.

Proceedings
[2] Baker, Peter J.; Kobayashi, Soma; Numata, Keiji, Chemoenzymatic Synthesis of Polypeptide- Based Functional Materials, Abstracts, 38th Northeast Regional Meeting of the American Chemical Society, Rochester, NY, United States, Sept. 30-Oct. 3. Pages NERM-203, 2012.
[1] Keiji Numata, Chemoenzymatic Syntheses of Poly(L-alanine) to Develop Spider Silk Mimics, Polymer Preprints (American Chemical Society, Division of Polymer Chemistry), Volume 53, Issue 2, pp336-337, 2012.

総説等（国内）
[13] 沼田圭司*. 「リグニンおよびその誘導体を利用したバイオマスプラスチックの微生物合成」、触媒学会　触媒、2016年58巻2号.
[12] 沼田圭司*. 「深海微生物によるバイオプラスチック生産」、現代科学、2016年1月号.
[11] 沼田圭司*. 「構造タンパク質の基礎物性を理解する −構造材料への利用に向けて−」、高分子学会、高分子、2015年12月号.
[10] 沼田圭司*. 「植物ミトコンドリアへの選択的遺伝子導入方法の開発」、バイオサイエンスとインダストリー、バイオインダストリー協会、73巻4号、2015.
[9] 沼田圭司*. 「ポリペプチドの化学酵素重合および機能材料への応用」、高分子学会超分子研究会、超分子アニュアルレビューNo.35、2015.
[8] 沼田圭司*. 「植物を利用したバイオプラスチック合成」、植物化学調節学会、植物の生長調節2014年12月、Vol49、No2.
[7] 沼田圭司*.　 Hot Topics「A new peptide synthesis via chemo-enzymatic reactions」高分子、2014年9月号.
[6] 沼田圭司*.　 Hot Topics「Biosynthesis of poly- and oligo-hydroxyalkanoate in marine bacteria.」高分子、2014年8月号.
[5] 沼田圭司*、富澤哲. 「リグニン構成成分を原料としたバイオプラスチックの微生物合成」、紙パルプ技術タイムス2014年8月号
[4] 沼田圭司*. 「酵素触媒によるバイオマスからのバイオポリマー合成とその展望」、日本油化学会誌 オレオサイエンス、2014年第14巻第3号.
[3] 沼田圭司*. 「二酸化炭素からバイオプラスチックを合成〜植物による化成品生産を可能にする植物遺伝子導入技術〜」月刊プラスチック、2014年3号
[2] 沼田圭司*. 「植物による物質生産を目指した新規植物遺伝子導入技術ペプチド法の開発」 化学経済, 2013年11月号.
[1] 沼田圭司*「生体触媒によるバイオ物質生産技術の創成」フジサンケイビジネスアイ、2013年5月16日.

Invited lecture & seminar

  1. [48] ○沼田圭司、New insights into spider dragline spinning. 日本化学会アジア国際シンポジウム（高分子部門）慶應大学日吉キャンパス、2017年3月18日.
  2. [47] ○Keiji Numata. Peptide-based transformation technologies for plant organelles. Gordon Research Conference 2017 “Chloroplast Biotechnology”, Ventura, CA, January 8-13, 2017.
  3. [46] ○沼田圭司、「ゲノム編集技術をいかに植物に利用するか」、日本農業経営大学校 特別講義、大手町サンケイプラザ 会議室、2016年12月8日.
  4. [45] ○沼田圭司、「人工シルクをいかに設計・合成するか　〜その課題と現状〜」、第２回社会に貢献する理研の工学研究シンポジウム、理化学研究所、2016年11月17日.
  5. [44] ○Keiji Numata. Silk-based structural materials inspired by hierarchical structures and functions of spider silk. Beihang University Advanced Materials Series, November 2, 2016.
    [43] ○Keiji Numata. Gene/protein delivery using peptide-based carriers to edit organellar and nuclear genomes. 2016 International Symposium on Plant Transformation Biotechnologies, Institute of Plant and Microbial Biology (IPMB), Academia Sinica, Taipei, October 17-19, 2016.
    [42] ○沼田圭司、ペプチドによるタンパク質導入技術を利用したゲノム編集、第８０回植物学会シンポジウム「植物から菌まで〜多様な生命の謎を探り生かすGenome Editing」、沖縄コンベンションセンター、2016年9月16-19日.
    [41] ○沼田圭司、Spider silk-like polypeptides: Enzymatic synthesis and materials design with water molecules. 高分子学会2016年度討論会日韓セッション若手講演、神奈川大学、2016年9月14-16日.
    [40] ○沼田圭司、Hierarchical structures and functions of spider silk.日本蜘蛛学会大会 公開シンポジウム、東京大学柏キャンパス、2016年8月20日.
    [39] ○沼田圭司、構造材料を志向したポリペプチドの生合成と材料設計、関東高分子若手研究会サマーキャンプ、東京農工大館山荘、2016年8月4-6日.
    [38] ○沼田圭司、シルク素材のタフネスおよび熱的安定性を向上させる技術を開発-水分子が引き起こすシルクの構造変化を解明-、理研イブニングセミナー、理研東京事務所、2016年6月22日.
    [37] ○Keiji Numata. Chemoenzymatic synthesis and material design of polypeptides. The 3rd International Symposium for Green-Innovation Polymers (GRIP2016), JAIST, March 4-5th, 2016.
    [36] ○土屋康佑、沼田圭司、酵素重合を利用した構造タンパク材料の開発、平成27年度日本素材物性学会研究会、秋田大学ベンチャー･ビジネス･ラボラトリー、2016年2月12日
    [35] ○沼田圭司、アミノ酸から構成される高分子の合成および構造材料への展開、高分子学会埼玉地区懇話会、リンテック（株）大会議場、2016年1月22日.
    [34] ○Keiji Numata. Potential as structural materials and chemo-enzymatic synthesis of poly(amino acid), Pacifichem2015, Hawaii, U.S.A, December 15-20, 2015.
    [33] ○沼田圭司、植物ミトコンドリアへの選択的遺伝子導入方法の開発: 高分子および高付加価値ケミカルの生合成への展開、JBA植物バイオ研究会（八丁堀）、2015年11月24日.
    [32] ○沼田圭司、ペプチドを利用した新たな遺伝子導入技術「ペプチド法」の開発、かずさDNA研究所セミナー、2015年11月2日.
    [31] ○Keiji Numata. Silk and Related Peptides as Structural Materials, 5TH MOLECULAR MATERIALS MEETING (M3) @ SINGAPORE, August 3-5, 2015.
    [30] ○Keiji Numata. Organelle-targeted gene delivery using peptide-based carriers, The 13th International Conference of Polymers for Advanced Technologies (PAT2015), Zhejiang University (Hangzhou, China), June 25-28, 2015.
    [29] ○Mieko Higuchi-Takeuchi & Keiji Numata. Bioplastic production by phototrophic bacteria using CO2. 7th International Conference on Green and Sustainable Chemistry (GSC-7) and 4th JACI/GSC Symposium. Tokyo, July 7, 2015.
    [28] ○Kenjiro Yazawa & Keiji Numata. Thermal property of silk fibroin: relationship between glass transition temperatures and water molecules. 7th International Conference on Green and Sustainable Chemistry (GSC-7) and 4th JACI/GSC Symposium. Tokyo, July 7, 2015.
    [27] ○Keiji Numata. Peptide-based gene carriers for organelle-targeted delivery system, 2015 Symposium for the promotion of applied research collaboration in Asia (SPARCA 2015), Taipei, Taiwan, February 8-11, 2015.
    [26] ○沼田圭司、ペプチド法による植物への遺伝子導入、植物ゲノム編集ワークショップ、岡山大学植物研、2014年11月4日.
    [25] ○沼田圭司、ペプチドの化学酵素合成およびバイオマス材料としての応用、第4回CSJ化学フェスタ2014、日本化学会、2014年10月15日.
    [24] ○Keiji Numata. Structural analysis of silk fibers and importance of the beta-sheet formation. International Silk Conference, Fudan University, Shanghai, October 8th, 2014.
    [23] ○沼田圭司、リグニンを利用した生分解性プラスチックの開発とバイオリファイナリーへの展開、～難利用木質バイオマス徹底活用～リグニン産業最前線 2014、サイエンス＆テクノロジー株式会社、2014年9月18日.
    [22] ○沼田圭司、ペプチドと核酸の複合体を利用した植物への遺伝子導入法の開発、新学術領域「植物細胞壁機能」セミナー、奈良先端科学技術大学院大学、2014年7月14日.
    [21] ○沼田圭司、Synthesis of poly- and oligo(hydroxyalkanoate)s by deep-sea bacteria, Colwellia spp., Moritella spp., and Shewanella spp.（受賞講演）、第63回高分子学会年次大会、名古屋国際会議場、2014年5月.
    [20] ○Nicholas Thomson, Jo-Ann Chuah, Keiji Numata. Increasing the range and performance of polyhydroxyalkanoates. ABS - 1512 – ICA, (18) Hybrids including Biomass based Materials, International Union of Materials Research Society- International Conference in Asia 2013, 19-12-2013.
    [19] ○Keiji Numata. Peptides for Green Innovations. Chinese Academy of Sciences. Invited Seminar, November 7th, 2013
    [18] ○Jose Manuel Ageitos, Keiji Numata. Protease-mediated synthesis of peptides in aqueous solution. Chinese Academy of Sciences. Invited Seminar, November 7th, 2013
    [17] ○沼田圭司、酵素で高分子を合成する、バイオマスイノベーション若手の会第一回キックオフワークショップ、東京大学、2013年11月6日.
    [16] ○沼田圭司、バイオマスからの新規プラスチック生産戦略―高分子科学の視点で挑む「二酸化炭素の資源化」および「バイオマスコンビナートと次世代農業の融 合」―、近畿バイオインダストリー振興会議バイオマス研究会公開セミナー、大阪科学技術センター、2013年9月4日.
    [15] ○Keiji Numata. Silk hydrogel with high strength and biocompatibility. Tissue Engineering and Regenerative Medicine International Society – Asia Pacific Chapter (TERMIS-AP) 2013 annual conference, Shanghai and Wuzhen, PR China, 25 October 2013.
    [14] ○沼田圭司、Biosynthesis and agricultural applications of biopolymers: Recent update on silk and bioplastics、先端生命科学研究所セミナー、慶応義塾大学、2013年7月.
    [13] ○沼田圭司、環境・植物工学への応用を目指したバイオポリマー設計、九州大学、2013年4月.
    [12] ○沼田圭司、植物による物質生産を目指した新規遺伝子導入「ペプチド法」、第１９回C-Bioセミナー、宇都宮大学、2013年1月.
    [11] ○沼田圭司、酵素触媒を用いた新規バイオポリマー合成、植物科学シンポジウム　植物最先端研究への期待、品川、コクヨホール、2012年12月3日.
    [10] ○沼田圭司、バイオポリエステルおよびバイオポリアミドの生合成とその応用、エコマテリアル研究会、東京、2012年7月.
    [9] ○沼田圭司、バイオポリマーの生合成およびグリーンイノベーションへの利活用、播磨理研SPring-8セミナー、2012年5月.
    [8] ○沼田圭司、ペプチドポリマーによるソフトマテリアルデザイン、第二回新規材料創成を目指した合成生物学、横浜、2012年1月.
    [7] ○Keiji Numata. Material design of silk polymers for hydrogel and nanoparticle. The 3rd International Conference on Biobased Polymers (ICBP2011), China, October 2011.
    [6] ○Keiji Numata. Recent update from RIKEN Enzyme Research Team, Royal Institute of Technology, Sweden, October 2011.
    [5] ○Keiji Numata, Yoshiharu Doi. Production and Material Design of Bio-Based Polyesters Engineering Plastic 2011, China, August 2011.
    [4] ○Keiji Numata, Yoshiharu Doi. Bio-based and biodegradable silk protein-bacterial polyester hybrid materials. Pacifichem 2010, 854, U.S.A., December 2010.
    [3] ○Keiji Numata, Yoshiharu Doi. Microbial production and biodegradation of polyhydroxyalkanoates. Pacifichem 2010, 847, U.S.A., December 2010.
    [2] ○Keiji Numata. Biosyntheses of functional polymers for various applications. RIKEN Plant Science Center Retreat, Japan, October 2010.
    [1] ○Keiji Numata, Masahiro Fujita, Yoshiharu Doi. Adsorption and hydrolysis mechanisms of PHA depolymerases. The 12th International Symposium on Biological Polyesters, 11A, Germany, October 2010.

 

Grants & funding

[21] 戦略的創造研究推進事業・総括実施型研究（ERATO） 沼田オルガネラ反応クラスタープロジェクト（代表 沼田圭司）、平成28年度10月〜平成33年度3月.
[20] 革新的研究開発推進プログラム（ImPACT）豊かで安全な社会と新しいバイオものづくりを実現する 人工細胞リアクタ（PM　野地博行）、プロジェクト：核酸運搬ペプチドを用いた微生物へのゲノムサイズDNA送達技術開発（吉積毅）、平成28年〜30年度.
[19] 科研費助成金 基盤研究(C)　H28-30年度 研究課題名：光合成細菌を利用した二酸化炭素と窒素固定反応によるPHA生産（代表：樋口美栄子）
[18] 革新的研究開発推進プログラム（ImPACT）セレンディピティの計画的創出による新価値創造（PM　合田圭介）、プロジェクト8チーム5：バイオ高分子を高効率に生産する光合成細菌の開発（代表　沼田圭司）、平成28年度〜30年度.
[17] 松籟科学技術振興財団 研究助成　H28.3-30年3月 研究課題名：植物を宿主とした有用物質大量生産に向けた代謝経路設計法の開発（代表：吉積毅）
[16] 革新的研究開発推進プログラム（ImPACT）超高機能構造タンパク質による素材産業革命（PM　鈴木隆領）、プロジェクト：天然高機能タンパク質素材の網羅的解析と高機能発現メカニズムの解明（プロジェクトリーダー　沼田圭司）、平成26年〜30年.
[15] 戦略イノベーション創造プログラム（SIP）（次世代農林水産業創造技術）、ゲノム編集技術と開花促進技術の普及と高度化（PD 西尾健）、平成26年〜30年、一過性モジュールによる外来遺伝子を残さない植物ゲノム編集技術の開発（代表　沼田圭司）.
[14] National Science Foundation 2014年度East Asia and Pacific Summer Institutes (EAPSI) プログラムH26 JSPSサマープログラム 調査研究費（代表：沼田圭司）.
[13] （継続）NEDO 平成23年度　先導的産業技術創出事業（若手研究グラント）プロジェクトＩＤ：11B12007d選択的オルガネラ形質転換法の開発によるバイオ物質の大量生産（H27まで継続）（代表：沼田圭司）.
[12] 科研費助成金 若手研究(B)　課題番号：25810110 H25-27年度 研究課題名：植物細胞壁を模倣したシルク-ペクチンハイドロゲルの創成とサンゴ再生基盤への利用（代表：沼田圭司）
[11] APEX DELIVERING EXCELLENCE GRANT 2012 (Malaysia), (代表 Kumar Sudesh、分担 Keiji Numata) Development of New Bio-Based Polymeric Materials Using Modified PHA Synthases for a Sustainable Tomorrow.  (2012年11月〜2014年8月).
[10] 理化学研究所 理事長ファンド若手研究会（代表 持田恵一、分担 沼田圭司）, 2012－2013年度.
[9] NEDO 平成23年度　先導的産業技術創出事業（若手研究グラント）プロジェクトＩＤ：11B12007d選択的オルガネラ形質転換法の開発によるバイオ物質の大量生産（H23-H25）（代表：沼田圭司）
[8] JST先端的低炭素化技術開発事業（ALCA）FS H23年度　課題番号J2011401、植物でのPHA生産、PHAの生産量計測、植物の代謝シュミレーション（代表 松井南、分担 沼田圭司）
[7] 理化学研究所 理事長ファンド若手研究会（代表 大谷美沙都、分担 沼田圭司）, 2011－2012年度.
[6] 科研費助成金 若手研究(B)　課題番号：23750264 H23-24年度　研究課題名：乳癌細胞をターゲットとした複数の機能性ペプチドから成る遺伝子ベクターの開発（沼田圭司）
[5] 住友財団　環境研究助成　プラヌラ幼生および微細藻類の人工共生系の構築を目的としたバイオポリマー材料の開発（沼田圭司）,理化学研究所，2011-2012年.
[4] 理化学研究所 理事長ファンド若手研究会、代表：沼田圭司、2010－2011年度.
[3] JSPS Grant for 59th Meeting of Nobel Laureates in Lindau dedicated to Chemistry, June 28th-July 3rd 2009.
[2] 科学研究費補助金（海外特別研究員奨励費）2008-2009年度、遺伝子治療による骨再生を目的とした低炎症性および生分解性インプラントの創製、代表：沼田圭司
[1] 科学研究費補助金（特別研究員奨励費）2006-2007年度、生分解性ポリエステルの結晶および鎖における酵素分解機構の解明、代表：沼田圭司、研究課題番号：06J05679
[その他]  企業との共同研究契約による研究費（国外1社、国内2社）

Awards
[19] Keiji Numata　The most highly prolific authors for Biomacromolecules, American Chemical Society (2016) link
[18] Keiji Numata　13th Annual Meeting of Science and Technology in Society forum (STS forum), Future Leaders参加（2016年）
[17] Nur Alia Oktaviani　Young Investigator Award, “Molecular conformation and dynamics of soluble precursor of recombinant repetitive domain of spider silk at different pH” XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016), August 2016.
[16] 沼田圭司　高分子学会 バイオ・高分子研究会 若手研究者奨励講演賞（2015年）
[15] 沼田圭司　高分子学会第61回高分子研究発表会（関西支部）ヤングサイエンティスト講演賞（2015年）
[14] Jo-Ann Chuah　RIKEN CSRS Incentive Award, “Peptide-derived gene carrier for mitochondrial-targeted delivery: from plants to mammalian cells” April 2015.
[13] Yoke-Ming Wong　Student Poster Presentation Award, “Optimization of poly(N-isopropylacrylamide) microgels exhibit enzyme-like amidase activity” Symposium for the promotion of applied research collaboration in Asia (SPARCA 2015), Taipei, Taiwan, February 10, 2015.
[12] 沼田圭司　Genomic Sciences Research Complex（GSC）七夕ミーティング2014フェロー（2014年）
[11] 沼田圭司　高分子学会 Polymer Journal 論文賞-日本ゼオン賞（2014年）
[10] 沼田圭司　高分子学会 高分子研究奨励賞 (2014年)
[9] Jose Ageitos　RIKEN BMEP Retreat Best Poster Award, “Proteinase K mediated synthesis of peptides” December 2013.
[8] Satoshi Tomizawa　RIKEN BMEP Retreat Best Poster Award, “Bacterial production of polyhydroxyalkanoate from aromatic carboxylic acid or sugar as a carbon source” December 2013.
[7] Jo-Ann Chuah　RIKEN BMEP Retreat Best Poster Award, “Transient Transformation of Plant Mitochondria using Mitochondria Targeting and Cell Penetrating Peptides” December 2013.
[6] 沼田圭司　平成23年度 理化学研究所 研究奨励賞 (2012年).
[5] 沼田圭司　リンダウノーベル賞受賞者会議 派遣 (2009年).
[4] 沼田圭司　高分子学会 関東高分子若手会 最優秀発表賞 博士の部 (2008年).
[3] 沼田圭司　東京工業大学大学院物質科学創造専攻 土肥賞（博士）(2008年).
[2] 沼田圭司　Poster Award of Joint Meeting of the 1st Asian-Oceanian Conference on Green and Sustainable Chemistry and the 7th Annual Green and Sustainable Chemistry Symposium (2007年).
[1] 沼田圭司　東京工業大学大学院物質科学創造専攻 土肥賞（修士）(2005年).