Publications | Previous works | Proceedings | 総説等(国内) | Invited lecture & seminar | Grants & funding | Members’ Awards


  1. Prashant G. Gudeangadi, Kei Uchida, Ayaka Tateishi, Kayo Terada, Hiroyasu Masunaga, Kousuke Tsuchiya*, Hitoshi Miyakawa, Keiji Numata*. Poly(alanine-nylon-alanine) as a bioplastic: chemoenzymatic synthesis, thermal properties and biological degradation effects. Polymer Chemistry, accepted.
  2. Hiromitsu Sogawa#, Kyohei Nakano#, Ayaka Tateishi, Keisuke Tajima, Keiji Numata*. Surface analysis of native spider draglines by FE-SEM and XPS. Frontiers in Bioengneering and Biotechnology, accepted.
  3. Kenjiro Yazawa, Ali D. Malay, Hiroyasu Masunaga, Y. Norma-Rashid, Keiji Numata*. Simultaneous effect of strain rate and humidity on the structure and mechanical behavior of spider silk. Communications Materials, 1: 10, 2020.
    Open access
    Press Release: 湿度と伸長速度によって変化するクモ糸の構造を解明
  4. Kousuke Tsuchiya*, Neval Yilmaz, Takaaki Miyamoto, Hiroyasu Masunaga, Keiji Numata*. Zwitterionic polypeptides: chemoenzymatic synthesis and loosening function for cellulose crystals. Biomacromolecules, accepted.
    Open access
  5. Michihiro Sugahara,* Koji Motomura, Mamoru Suzuki, Tetsuya Masuda, Yasumasa Joti, Keiji Numata, Kensuke Tono, Makina Yabashi, Tetsuya Ishikawa. Viscosity-adjustable grease matrices for serial nanocrystallography. Scientific Reports, 10:1371, 2020.
    Open access
  6. Hiromitsu Sogawa, Treratanakulwongs Korawit, Hiroyasu Masunaga, Keiji Numata*. Silk/natural rubber (NR) and 3,4-dihydroxyphenylalanine (DOPA)-modified silk/NR composites: synthesis, secondary structure, and mechanical properties. Molecules, Special issue "Silk Fibroin Materials", 25(1), 235, 2020.
    Open access
  7. Masaki Odahara*, Yoko Horii, Mitsuhiro Kimura, Keiji Numata*. Efficient callus induction and completion of regeneration in kenaf Hibiscus cannabinus. Plant Biotechnology, accepted.
  8. Akira Matsumoto*, Keiji Numata*. Biomaterials Science and Engineering in Japan: attractive, multidisciplinary, and essential research field in Japan. ACS Biomaterials Science and Engineering, 5, 11, 5559-55560, 2019.
  9. Shota Kato, Kazunari Ozasa, Mizuo Maeda, Yuri Tanno, Mieko Higuchi-Takeuchi, Keiji Numata, Yutaka Kodama, Mayuko Sato, Kiminori Toyooka, Tomoko Shinomura*. Carotenoids are essential for light perception by the eyespot apparatus to initiate the phototactic movement of Euglena gracilis. Plant Journal. Accepted.
    Open access
  10. Cooper J. Love, Bogdan A. Serban, Takuya Katashima, Keiji Numata, Monica A. Serban*. Mechanistic insights into silk fibroin’s adhesive properties via chemical functionalization of serine side chains. ACS Biomaterials Science & Engineering. 5, 5960-5967, 2019.
  11. Kousuke Tsuchiya*, Keiji Numata*. Facile Terminal Functionalization of Peptides by Protease-Catalyzed Chemoenzymatic Polymerization Toward Synthesis of Polymeric Architectures Consisting of Peptides. Polymer Chemistry, 11, 560-567, 2020.
    Open access
  12. Mieko Takeuchi-Higuchi*, Kumiko Morisaki, Keiji Numata*. Facile transformation method of marine purple photosynthetic bacteria using chemically competent cells. MicrobiologyOpen.2019;00:e953.
    Open access
  13. Chonprakun Thagun, Jo-Ann Chuah, Keiji Numata*. Targeted gene delivery into various plastids mediated by clustered cell-penetrating and chloroplast-targeting peptides. Advanced Science, 1902064, 2019.
    Open access
    Back Cover
    Press Release: ペプチドによる色素体の改変 (JSTプレス)
  14. Mieko Higuchi-Takeuchi*, Keiji Numata*. Marine purple photosynthetic bacteria as sustainable microbial production hosts (Invited mini-review). Frontiers in Bioengineering and Biotechnology, Vol 7, 258, 2019.
    Open access
  15. Neval Yilmaz, Yutaka Kodama, Keiji Numata*. Revealing the architecture of cell wall in living plant cells by bio-imaging and enzymatic degradation. Biomacromolecules, Accepted.
    Open access
  16. Boyang Guo, Jun Itami, Kazusato Oikawa, Yoko Motaoda, Takanori Kigawa, Keiji Numata*. Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides. Plos One, e0214033, 2019.
    Open access
  17. Kang Yang, Juan Guan*, Keiji Numata, Change Wu, Sujun Wu, Zhengzhong Shao, Robert O. Ritchie*. Integrating tough Antheraea pernyi silk and strong carbon fibres for impact-critical structural composites. Nature Communications. 10, 3786, 2019.
    Open access
  18. Kousuke Tsuchiya,* Naruki Kurokawa, Joan Gimenez-Dejoz, Prashant G. Gudeangadi, Hiroyasu Masunaga, Keiji Numata*. Periodic introduction of aromatic units in polypeptides via chemoenzymatic polymerization giving specific secondary structures with high thermal stability. Polymer Journal. 51, 1287–1298, 2019.
    Open access
    Front Cover
  19. Nur Alia Oktaviani, Akimasa Matsugami, Fumiaki Hayashi, Keiji Numata*. Ion effects on conformation and dynamics of repetitive domain of spider silk protein: Implication on solubility and β-sheet formation. Chemical Communications. 55, 9761-9764, 2019.
    Open access
    Inside Front Cover
    Press Release: クモ糸の紡糸過程におけるイオンの効果を解明 (English ver.)
  20. Kang Yang, Kenjiro Yazawa, Kousuke Tsuchiya, Keiji Numata,* Juan Guan*. Molecular interactions and toughening mechanisms in silk fibroin-epoxy resin blend films. Biomacromolecules, 20(6), 2295-2304, 2019.
  21. Joan Gimenez-Dejoz, Kousuke Tsuchiya, Keiji Numata*. Quantum mechanics/molecular mechanics (QM/MM) simulation studies on the papain-mediated chemoenzymatic polymerization. ACS Chemical Biology, 14(6) 1280-1292, 2019.
    Open access
    Front Cover
  22. Mieko Higuchi-Takeuchi*, Keiji Numata*. Acetate-inducing metabolic states enhance polyhydroxyalkanoate production of marine purple nonsulfur bacteria under an aerobic condition. Frontiers in Bioengineering and Biotechnology,7, 118, 2019.
    Open access
  23. Md Monirul Islam, Masaki Odahara, Takeshi Yoshizumi, Kazusato Oikawa, Mitsuhiro Kimura, Masayuki Su’etsugu, Keiji Numata*. Cell-penetrating peptide-mediated transformation of large plasmid DNA into Escherichia coli. ACS Synthetic Biology, 8(5), 1215-1218, 2019.
    Open access
    Inside Cover
    Press Release: 機能性ペプチドによる巨大DNAの細胞内導入
  24. Choon Pin Foong#, Mieko Higuchi-Takeuchi#, Keiji Numata*. Optimal iron concentrations for growth-associated polyhydroxyalkanoate biosynthesis in the marine photosynthetic purple bacterium Rhodovulum sulfidophilum under photoheterotrophic condition. Plos One,14 (4), e0212654, 2019.
    Open access
  25. Nobuaki Kono, Hiroyuki Nakamura, Rintaro Ohtoshi, Masaru Tomita, Keiji Numata, Kazuharu Arakawa*, The bagworm genome reveals a unique fibroin gene that provides high tensile strength. Communications Biology, 2 (1), 148, 2019.
    Open access
    Press Release: ミノムシのゲノム解明ーミノムシが魅せる糸の強さに関する遺伝子配列の特性を発見ー
  26. Kousuke Tsuchiya, Yu Miyagi, Takaaki Miyamoto, Prashant G. Gudeangadi, Keiji Numata*. Chapter 8 Synthesis of Polypeptides. “Enzymatic Polymerization towards Green Polymer Chemistry”, Springer, 2019.
  27. Hiromitsu Sogawa, Nao Ifuku, and Keiji Numata*, 3,4-Dihydroxyphenylalanine (DOPA)-containing silk fibroin: its enzymatic synthesis and adhesion properties. ACS Biomaterials Science & Engineering,Vol 5, 5644-5651, 2019.
    Open access
    Inside Cover
    Press Release: シルクを接着剤にする
  28. Takuya Katashima#, Ali D. Malay#, Keiji Numata*. Chemical modification and biosynthesis of silk-like polymers (Invited mini-review). Current Opinion in Chemical Engineering,Volume 24, pp61-68, 2019.
    Open access
  29. Keiko Midorikawa, Yutaka Kodama*, Keiji Numata*. Vacuum/Compression Infiltration-mediated Permeation Pathway of a Peptide-pDNA Complex as a Non-Viral Carrier for Gene Delivery in Planta. Scientific Reports, 9, 271, 2019.
    Open access
    Press Release: ペプチドによる遺伝子送達には植物の構造的脆弱性が重要
  30. Kousuke Tsuchiya*, Nao Ifuku, Yasuhito Koyama, Keiji Numata*. Development of regenerated silk films coated with fluorinated polypeptides to achieve high water repellency and biodegradability in seawater. Polymer Degradation and Stability,Volume 160, 96-101, 2019.
    Open access
  31. Mitsuhiro Kimura*, Takeshi Yoshizumi, Keiji Numata*, A centrifugation-assisted peptide-mediated gene transfer method for various high-throughput analyses. Plant Biotechnology, Volume 36, Issue 1 pp49-52, 2019.
    Open access
  32. Takaaki Miyamoto, Kousuke Tsuchiya*, Keiji Numata*, Block copolymer/plasmid DNA micelles postmodified with functional peptides via thiol-maleimide conjugation for efficient gene delivery into plants. Biomacromolecules, Volume 20 (2), 653-661, 2019.
    Open access
    Inside Cover
  33. Kazusato Oikawa#, Md Monirul Islam#, Yoko Horii, Takeshi Yoshizumi, Keiji Numata*, Screening of a cell-penetrating peptide library in Escherichia coli: relationship between cell penetration efficiency and cytotoxicity. ACS Omega, 3, 16489-16499, 2018.
    Open access
  34. Dongshi Zhang, Wonsuk Choi, Kenjiro Yazawa, Keiji Numata, Ayaka Tateishi, Sung-Hak Cho, Hsiu-Pen Lin, Yaw Kuen Li, Yoshihiro Ito, Koji Sugioka*. Two Birds with One Stone: Spontaneous Size Separation and Growth Inhibition of Femtosecond Laser-Generated Surfactant-Free Metallic Nanoparticles via Ex Situ SU-8 Functionalization. ACS Omega, 3, 10953-10966, 2018.
    Open access
  35. Chris Holland*, Keiji Numata*, Jelena Rnjak-Kovacina*, Philipp Seib*. The biomedical use of silk: past, present, future. Advanced Healthcare Materials, 1800465, 2018.
    Open access
  36. Kosuke Tsuchiya*, Keiji Numata*. Protease-Catalyzed Polymerization of Tripeptide Esters Containing Unnatural Amino Acids: α,α-Disubstituted and N-Alkylated Amino Acids. Green Polymer Chemistry: New Products, Processes, and Applications. ACS Symposium Series, Chapter 7, pp 95-105, 2018.
    Open access
  37. Kenjiro Yazawa#, Ali D. Malay#, Hiroyasu Masunaga, Keiji Numata*, Role of Skin Layers on Mechanical Properties and Supercontraction of Spider Dragline Silk Fiber. Macromolecular Bioscience, Special Issue 65th birthday for David Kaplan, 1800220, 2018.
    Open access
    Front Cover
  38. Erik R. Johnston, Yu Miyagi, Jo-Ann Chuah, Keiji Numata, Monica A. Serban*, The interplay between silk fibroin’s structure and its adhesive properties. ACS Biomaterials Science & Engineering, Volume 4 (8), 2815-2824, 2018
  39. Keiji Numata*, Yoko Horii, Kazusato Oikawa, Yu Miyagi, Taku Demura, Misato Ohtani, Library screening of cell-penetrating peptide for BY-2 cells, leaves of Arabidopsis, tobacco, tomato, poplar, and rice callus. Scientific Reports, 8, 10966, 2018.
    Open access
    Press Release: 多様な植物に侵入するペプチドの探索
  40. Nur Alia Oktaviani, Akimasa Matsugami, Ali D. Malay, Fumiaki Hayashi, David L. Kaplan, Keiji Numata*, Conformation and dynamics of soluble repetitive domain elucidates the initial β-sheet formation of spider silk. Nature Communications, 9:2121, 2018.
    Open access
    Press Release: クモ糸が形成される初期機構を解明 (JSTプレス)
    Press Release: Scientists discover key mechanism behind the formation of spider silk
  41. Kenjiro Yazawa#, Ali D. Malay#, Nao Ifuku, Takaoki Ishii, Hiroyasu Masunaga, Keiji Numata*, Combination of amorphous silk fiber spinning and post-spinning crystallization gives regenerated tough silk fibers. Biomacromolecules, Volume 19(6), pp2227–2237, 2018.
    Open access
  42. Keiji Numata*, Nao Ifuku, Akira Isogai. Silk composite with a fluoropolymer as a water-resistant protein-based material. Polymers, Special issue on Protein Biopolymer,10(4), pp459, 2018.
    Open access
  43. Takeshi Yoshizumi*, Kazusato Oikawa, Jo-Ann Chuah, Yutaka Kodama, Keiji Numata*. Selective gene delivery for integrating exogenous DNA into plastid and mitochondrial genomes using peptide-DNA complexes. Biomacromolecules, Volume 19(5), pp1582–1591, 2018.
    Open access
  44. Prashant G. Gudeangadi, Kousuke Tsuchiya*, Takamasa Sakai, Keiji Numata*, Chemoenzymatic Synthesis of Polypeptides Consisting of Periodic Di- and Tri-peptide Motifs Similar to Elastin. Polymer Chemistry, Volume 9, pp2336 – 2344, 2018.
    Open access
  45. Jo-Ann Chuah, Keiji Numata*, Stimulus-responsive peptide for effective delivery and release of DNA in plants. Biomacromolecules, 19 (4), pp1154–1163, 2018.
    Open access
    Front Cover
  46. Kousuke Tsuchiya*, Takaoki Ishii, Hiroyasu Masunaga, Keiji Numata*, Spider dragline silk composite films doped with linear and telechelic polyalanine: Effect of polyalanine on the crystal structure and mechanical properties. Scientific Reports, 8:3654, 2018.
    Open access
    Press Release: ペプチドでシルク素材を高強度化
  47. Noorhidayah-Mamat*, Kenjiro Yazawa, Keiji Numata, Y. Norma-Rashid, Morphological and mechanical properties of flexible resilin joints on damselfly wings (Rhinocypha spp.). Plos One, 13(3): e0193147, 2018.
    Open access
  48. Nicholas M. Thomson*, Tomokazu Shirai, Marco Chiapello, Akihiko Kondo, Krishna. J. Mukherjee, Easan Sivaniah, Keiji Numata, David K. Summers, Efficient 3-hydroxybutyrate production by quiescent Escherichia coli microbial cell factories is facilitated by indole-induced proteomic and metabolomic changes. Biotechnology Journal, 13(5), 1700571, 2018.
  49. Ali D. Malay*, Kazuharu Arakawa, Keiji Numata*, Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins. PLoS ONE, 12(8): e0183397, 2017.
    Open access
  50. Mieko Higuchi-Takeuchi, Yoko Motoda, Takanori Kigawa, Keiji Numata*, Class I polyhydroxyalkanoate synthase from the purple photosynthetic bacterium Rhodovulum sulfidophilum predominantly exists as a functional dimer in the absence of substrate. ACS Omega, Volume 2 (8), pp5071–5078, 2017.
    Open access
  51. Kenjiro Yazawa, Joan Gimenez-Dejoz, Hiroyasu Masunaga, Takaaki Hikima, Keiji Numata*, Chemoenzymatic synthesis of a peptide containing nylon monomer units for thermally processable peptide material application. Polymer Chemistry, Volume 8, pp4172-4176, 2017.
    Open access
    Front Cover
  52. Kousuke Tsuchiya*, Keiji Numata*, Chemoenzymatic synthesis of polypeptides containing the unnatural amino acid 2-aminoisobutyric acid. Chemical Communications, Volume 53, pp7318–7321, 2017.
    Open access
  53. Keiji Numata*, Nao Ifuku, Hiroyasu Masunaga, Takaaki Hikima, Takamasa Sakai, Silk resin with hydrated dual chemical-physical crosslinks achieves high strength and toughness. Biomacromolecules, Volume 18(6), pp1937–1946, 2017.
    Open access
  54. Michihiro Sugahara*, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata. Hydroxyethyl cellulose matrix applied to serial crystallography. Scientific Reports, 7, 703, 2017.
    Open access
  55. Ting-Yu Lin, Hiroyasu Masunaga, Ryota Sato, Ali D. Malay, Kiminori Toyooka, Takaaki Hikima, Keiji Numata*. Liquid crystalline granules align in a hierarchical structure to produce spider dragline microfibrils. Biomacromolecules, Volume 18(4), pp1350–1355, 2017.
    Open access
  56. Tetsuya Masuda*, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Takanori Nakane, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Bunzo Mikami, Osamu Nureki, Keiji Numata, So Iwata, Michihiro Sugahara*, Atomic resolution structure of serine protease proteinase K by serial femtosecond crystallography. Scientific Reports, 7, 45604 , 2017.
    Open access
  57. Kousuke Tsuchiya*, Hiroyasu Masunaga, Keiji Numata*, Tensile reinforcement of silk films by the addition of telechelic-type polyalanine. Biomacromolecules, Volume 18(3), pp1002–1009, 2017.
    Open access
  58. Kousuke Tsuchiya*, Keiji Numata*, Chemical synthesis of multiblock copolypeptides inspired by spider dragline silk proteins. ACS Macro Letters, Volume 6, pp103–106, 2017.
    Open access
    Press Release: 化学的手法でクモの糸を創る
  59. Takeshi Yoshizumi, Miwa Yamada, Mieko Higuchi-Takeuchi, Ken’ichiro Matsumoto, Seiichi Taguchi, Minami Matsui*, Keiji Numata*, Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants. Plant Biotechnology, Volume 34, 1 pp39-43, 2017.
    Open access
  60. Chayatip Insomphun, Jo-Ann Chuah, Shingo Kobayashi, Tetsuya Fujiki, Keiji Numata*, Influence of hydroxyl groups on the cell viability of polyhydroxyalkanoate scaffolds for tissue engineering. ACS Biomaterials Science and Engineering,Volume 3, pp3064–3075, 2017.
    Open access
    Press Release: 生体親和性の高いバイオプラスチック
  61. Kosuke Tsuchiya*, Keiji Numata*. Chemoenzymatic synthesis of polypeptides as functional and structural materials (Feature article). Macromolecular Bioscience, Volume 17, pp1700177, 2017.
    Open access
    Front Cover
    Advanced Science News: Polypeptides as Structural Materials via Chemoenzymatic Synthesis.
  62. Jo-Ann Chuah, Akimasa Matsugami, Fumiaki Hayashi, Keiji Numata*, Self-Assembled Peptide-Based System for Mitochondrial-Targeted Gene Delivery: Functional and Structural Insights. Biomacromolecules,Volume 17(11), pp3547–3557, 2016.
    Open access
    Chemical & Engineering News: A step toward mitochondrial gene therapy.
  63. Keiji Numata*, Yoko Horii, Yoko Motoda, Tokumi Hirai, Chikako Nishitani, Satoru Watanabe, Takanori Kigawa, Yutaka Kodama, Direct introduction of neomycin phosphotransferase II protein into apple leaves to confer kanamycin resistance. Plant Biotechnology,Volume 33(5), pp403-407, 2016.
    Open access
  64. Yoke-Ming Wong, Hiroyasu Masunaga, Jo-Ann Chuah, Kumar Sudesh, Keiji Numata*, Enzyme-mimic peptide assembly to achieve amidolytic activity. Biomacromolecules, Volume 17(10), pp3375–3385, 2016.
    Open access
  65. Mieko Higuchi-Takeuchi, Kumiko Morisaki, Keiji Numata*. A screening method for the isolation of polyhydroxyalkanoate-producing purple non-sulfur photosynthetic bacteria from natural seawater. Frontiers in Microbiology, Special issue “Biotechnology of microalgae, based on molecular biology and biochemistry of eukaryotic algae and cyanobacteria”,7:1509, 2016.
    Open access
  66. Pei-Shze Mok, Diana Hooi-Ean Ch’ng, Soo-Peng Ong, Keiji Numata, Kumar Sudesh*, Characterization of the depolymerizing activity of commercial lipases and detection of lipase-like activities in animal organ extracts using poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thin film. AMB Express, 6:97, 2016.
    Open access
  67. Mieko Higuchi-Takeuchi, Kumiko Morisaki, Kiminori Toyooka, Keiji Numata*, Synthesis of high-molecular-weight polyhydroxyalkanoates by marine photosynthetic purple bacteria. PLOS ONE, 11(8): e0160981, 2016.
    Open access
    Press Release: 高分子量バイオプラスチックを生産する海洋性の光合成細菌
  68. Jo-Ann Chuah, Yoko Horii, Keiji Numata*, Peptide-Derived Method to Transport Genes and Proteins Across Cellular and Organellar Barriers in Plants. Journal of Visualized Experiments (JoVE), 118, e54972, 2016.
    Open access
  69. Ali D. Malay, Hiroe Watanabe, Kenjiro Yazawa, Ryota Sato, Nao Ifuku, Hiroyasu Masunaga, Takaaki Hikima, Juan Guan, Biman B. Mandal, Siriporn Damrongsakkul, Keiji Numata*. Relationships between physical properties and sequences in silkworm silks. Scientific Reports.6:27573, 2016.
    Open access
    Press Release: シルクの材料特性とアミノ酸配列の相関を解明English ver
  70. Kenjiro Yazawa, Keiji Numata*. Papain-catalyzed synthesis of peptides containing a nylon unit. Polymers, Special Issue "Enzymatic Polymer Synthesis", Volume 8(5), 194, 2016.
    Open access
  71. Kiaw Kiaw Ng, Yoko Motoda, Satoru Watanabe, Takanori Kigawa, Ahmad Sofiman Othman, Yutaka Kodama, Keiji Numata* Intracellular delivery of proteins in intact plant via fusion peptides. PLoS ONE. 11(4), e0154081, 2016.
    Open access
  72. Kenjiro Yazawa#, Michihiro Sugahara#, Katsuhide Yutani, Michiyo Takehira, and Keiji Numata*. Derivatization of Proteinase K with Heavy Atoms Enhances Its Thermal Stability. ACS Catalysis. Volume 6, pp3036–3046, 2016.
    Open access
  73. Sachiko Nitta*, Ayaka Komastu, Taisei Ishii, Hiroyuki Iwamoto, Keiji Numata*, Synthesis of peptides with narrow molecular weight distributions via exopeptidase-catalyzed aminolysis of hydrophobic amino acid alkyl esters. Polymer Journal. Volume 48, pp955-961, 2016.
    Open access
  74. Michihiro Sugahara*, Changyong Song, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Takanori Nakane, Fumiaki Yumoto, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, So Iwata. Oil-free hyaluronic acid matrix for serial femtosecond crystallography. Scientific Reports. 6, pp24484, 2016.
    Open access
  75. Chayatip Insomphun, Shingo Kobayashi, Tetsuya Fujiki, Keiji Numata*. Biosynthesis of polyhydroxyalkanoates containing hydroxyl group from glycolate in Escherichia coli. AMB Express, 6 (1), pp1-8 2016.
    Open access
  76. Yinan Ma, Zhibo Li*, Keiji Numata*, Synthetic short peptides for rapid fabrication of monolayer cell sheets. ACS Biomaterials Science and Engineering. Volume 2 (4), pp697-706, 2016.
    Open access
  77. Kenjiro Yazawa, Kana Ishida, Hiroyasu Masunaga, Takaaki Hikima, Keiji Numata*, Influence of water content on the β-sheet formation, thermal stability, water removal, and mechanical properties of silk materials. Biomacromolecules. Volume 17 (3), pp1057-1066, 2016.
    Open access
  78. Kousuke Tsuchiya*, Keiji Numata*, Papain-Catalyzed Chemoenzymatic Synthesis of Telechelic Polypeptides Using Bis(leucine ethyl ester) Initiator. Macromolecular Bioscience.Volume 16, Issue 7, pp1001–1008, 2016 (Front Cover).
    Front Cover
  79. Leo Simmons, Kousuke Tsuchiya*, Keiji Numata*. Chemoenzymatic modification of silk fibroin with poly(2,6-dimethyl-1,5-phenylene ether) using horseradish peroxidase. RSC Advances. Volume 6, pp28737-28744, 2016.
    Open access
  80. Jose Manuel Ageitos, Kenjiro Yazawa, Ayaka Tateishi, Kosuke Tsuchiya, Keiji Numata*, Benzyl ester group of amino acid monomers broadens the substrate specificity and enhance the affinity of papain in chemo-enzymatic copolymerization. Biomacromolecules, Volume 17, pp314–323, 2016.
    Open access
  81. Yinan Ma, Ryota Sato, Zhibo Li*, Keiji Numata*, Chemoenzymatic synthesis of oligo(L-cysteine) for use as thermal stable bio-based material. Macromolecular Bioscience, Volume 16, pp151–159, 2016.
  82. Jose Manuel Ageitos, Jo-Ann Chuah, Keiji Numata* Design considerations for properties of nanocarriers on disposition and efficiency of drug and gene delivery. Nanomedicines: design, delivery and detection. Edited by Martin Braddock, Royal Society of Chemistry, 2016.
    Open access
  83. Keiji Numata*, Ryota Sato, Kenjiro Yazawa, Takaaki Hikima, Hiroyasu Masunaga. Crystal structure and physical properties of Antheraea yamamai silk fibers: long poly(alanine) sequences are partially in crystalline region. Polymer, Volume 77, pp87-94, 2015.
  84. Keiji Numata*, Hiroyasu Masunaga, Takaaki Hikima, Sono Sasaki, Kazuhide Sekiyama, Masaki Takata. Use of extension-deformation-based crystallization of silk fibres to differentiate their functions in nature. Soft Matter, Volume 11, pp6335-6342, 2015.
  85. Alex C. Levine, Angelina Sparano, Frederick F. Twigg, Keiji Numata, Christopher T. Nomura*. Influence of cross-linking on the physical properties and cytotoxicity of polyhydroxyalkanoate (PHA) scaffolds for tissue engineering. ACS Biomaterials Science & Engineering, Volume 1, pp567–576, 2015.
  86. Jose Manuel Ageitos, Jo-Ann Chuah, Keiji Numata*, Chemo-Enzymatic Synthesis of Linear and Branched Cationic Peptides: Evaluation as Gene Carriers, Macromolecular Bioscience, Volume 15, Issue 7, pp990–1003, 2015.
  87. Keiji Numata*, Kumiko Morisaki. Screening of marine bacteria to synthesize polyhydroxyalkanoate from lignin: Contribution of lignin derivatives to biosynthesis by Oceanimonas doudoroffii. ACS Sustainable Chemistry and Engineering, Volume 3, Issue 4, pp569–573, 2015.
    Open access
  88. Keiji Numata*, Yoko Motoda, Satoru Watanabe, Takashi Osanai, and Takanori Kigawa. Co-expression of two polyhydroxyalkanoate synthase subunits from Synechocystis sp. PCC 6803 by cell free synthesis and their specific activity for polymerization of 3-hydroxybutyryl-CoA. Biochemistry, Volume 54, Issue 6, pp1401–1407, 2015.
    Open access
  89. Yoke-Ming Wong, Yu Hoshino*, Kumar Sudesh, Yoshiko Miura, Keiji Numata*. Optimization of poly(N-isopropylacrylamide) as an artificial amidase. Biomacromolecules, Volume 16, Issue 1, pp411-421, 2015.
  90. Jo-Ann Chuah, Takeshi Yoshizumi, Yutaka Kodama, Keiji Numata*. Gene introduction into the mitochondria of Arabidopsis thaliana via peptide-based carriers. Scientific Reports, 5, 7751 2015.
    Open access
    Press Release: 植物ミトコンドリアへ選択的に遺伝子導入する手法を開発English ver.)
  91. Manoj Lakshmanan, Takeshi Yoshizumi, Kumar Sudesh, Yutaka Kodama, Keiji Numata*, Double stranded DNA introduction into intact plant using peptide-DNA complexes. Plant Biotechnology, Volume 32, Issue 1, pp39-45, 2015.
    Open access
  92. Keiji Numata*. Poly(amino acid)s/polypeptides as potential functional and structural materials (Focus review). Polymer Journal, Volume 47 Issue 8, pp537-545, 2015.
  93. Peter James Baker, Siddharth V Patwardhan, Keiji Numata*, Synthesis of Homopolypeptides by Aminolysis Mediated by Proteases Encapsulated in Silica Nanospheres. Macromolecular Bioscience, Volume 14, Issue 11, pp1619-26, 2014.
  94. Keiji Numata*, Peter James Baker, Synthesis of adhesive peptides similar to those found in blue mussel (Mytilus edulis) using papain and tyrosinase. Biomacromolecules, Volume 15, Issue 8, pp3206-12, 2014.
    Open access
  95. Satoshi Tomizawa, Jo-Ann Chuah, Keiji Matsumoto, Yoshiharu Doi, Keiji Numata* Understanding the limitations in the biosynthesis of polyhydroxyalkanoate (PHA) from lignin derivatives. ACS Sustainable Chemistry and Engineering, Volume 2, Issue 5, pp1106–1113, 2014.
    Open access
    Press Release: リグニン構成成分を原料としたバイオプラスチックの微生物生産
  96. Keiji Numata*, Misato Ohtani, Takeshi Yoshizumi, Taku Demura, Yutaka Kodama. Local gene silencing in plants via synthetic dsRNA and carrier peptide. Plant Biotechnology Journal (Wiley), Volume 12, Issue 8, pp1027-1034, 2014.
    Open access
  97. Kazunori Ushimaru, Yoko Motoda, Keiji Numata, Takeharu Tsuge*. Phasin Proteins Activate Aeromonas caviae Polyhydroxyalkanoate (PHA) Synthase but not Ralstonia eutropha PHA Synthase. Applied Environmental Microbiology, Volume 80, Issue 9, pp2867-2873, 2014.
  98. Takashi Osanai, Akira Oikawa, Keiji Numata, Ayuko Kuwahara, Hiroko Iijima, Yoshiharu Doi, Kazuki Saito, Masami Yokota Hirai*. Pathway-Level Acceleration of Glycogen Catabolism by Response Regulator Rre37 in the Cyanobacterium Synechocystis sp. PCC 6803. Plant Physiology, Volume 164, Volume 4, pp1831-1841, 2014.
  99. Jenny Fagerland, Anna Finne Wistrand, Keiji Numata*. Short one-pot chemo-enzymatic synthesis of L-lysine and L-alanine diblock cooligopeptides. Biomacromolecules, Volume 15, Issue 3, pp735-743, 2014.
  100. Keiji Numata*, Shoya Yamazaki, Jo-Ann Chuah, Takuya Katashima, Naofumi Naga, Takamasa Sakai. Silk-Pectin Hydrogel with Superior Mechanical Properties, Biodegradability, and Biocompatibility. Macromolecular Bioscience, Volume 14, Issue 6, pp799-806,2014 (Back cover)
    Back Cover
  101. Chayatip Insomphun, Jun Mifune, Izumi Orita, Keiji Numata, Satoshi Nakamura, Toshiaki Fukui*. Modification of ß-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil. Journal of Bioscience and Bioengineering, Volume 117, Issue 2, pp184-190,2014.
  102. Satoshi Tomizawa, Chuah Jo-Ann, Misato Ohtani, Taku Demura and Keiji Numata. Biosynthesis of Polyhydroxyalkanoate by Marine Bacteria Vibrio sp. Using Sugars, Plant Oil and Unsaturated Fatty Acids as a Sole Carbone Source. Green Polymer Chemistry: Biocatalysis and Materials II, Chapter 15, pp 211–221, ACS Symposium Series, Vol. 1144, ISBN13: 9780841228955 eISBN: 9780841228962, 2014.
  103. Kenjiro Yazawa, Keiji Numata*. Recent advances in the chemoenzymatic peptide syntheses. Molecules, Special Issue "Peptide Chemistry", Volume 19, Issue 9, pp13755-13774, 2014.
    Open access
  104. Jo-Ann Chuah, David Kaplan, Keiji Numata*. Engineering Peptide-based Carriers for Drug and Gene Delivery. Engineering in Translational Medicine. Springer, London (2014).
  105. Jose Manuel Ageitos, Peter James Baker, Michihiro Sugahara, Keiji Numata*. Proteinase K-catalyzed synthesis of linear and star oligo-L-phenylalanine conjugates. Biomacromolecules, Volume 14, Issue 10, pp3635-3642,2013.
    Open access
  106. Takashi Osanai, Keiji Numata, Akira Oikawa, Kiminori Toyooka, Mayuko Sato, Ayuko Kuwahara, Hiroko Iijima, Yoshiharu Doi, Kan Tanaka, Kazuki Saito, and Masami Yokota Hirai*. Overexpression of an RNA Polymerase Sigma Factor SigE Increases Bioplastic Production in Cyanobacteria. DNA Research, Volume 20, Issue 6, pp525-535,2013.
    Open access
  107. Koichiro Tachibana, Yuichi Urano, Keiji Numata*. Biodegradability of Nylon 4 Film in a Marine Environment. Polymer Degradation and Stability, Volume 98, Issue 9, pp1847–1851, 2013.
  108. Jo-Ann Chuah, Satoshi Tomizawa, Miwa Yamada, Takeharu Tsuge, Yoshiharu Doi, Kumar Sudesh*, Keiji Numata*. Characterization of Site-Specific Mutations in a Short-Chain-Length-Medium-Chain-Length Polyhydroxyalkanoate Synthase: in vivo and in vitro Studies on Enzymatic Activity and Substrate Specificity. Applied and Environmental Microbiology, Volume 79, Issue 12, pp3813-3821, 2013.
  109. Keiji Numata*, Kumiko Morisaki, Satoshi Tomizawa, Misato Ohtani, Taku Demura, Masayuki Miyazaki, Yuichi Nogi, Shigeru Deguchi and Yoshiharu Doi. Synthesis of Poly- and Oligo(hydroxyalkanoate)s by Deep-Sea Bacteria, Colwellia spp., Moritella spp. and Shewanella spp. Polymer Journal, Volume 45, pp1094-1100, 2013 (Selected as a Featured Article, October 2013).
  110. Miwa Yamada, Shuntaro Takahashi, Yoshio Okahata, Yoshiharu Doi, Keiji Numata*. Monitoring and Kinetic Analysis of the Molecular Interactions by which a Repressor Protein, PhaR, Binds to Target DNAs and Poly[(R)-3-hydroxybutyrate]. AMB express, Volume 3, 6, 2013.
    Open access
  111. Manoj Lakshmanan, Yutaka Kodama, Takeshi Yoshizumi, Kumar Sudesh, Keiji Numata*. Rapid and Efficient Gene Delivery into Plant Cells Using Designed Peptide Carriers. Biomacromolecules, Volume 14, Issue 1, pp10-16, 2013.
    Open access
  112. Jo-Ann Chuah, Miwa Yamada, Seiichi Taguchi, Sudesh Kumar, Yoshiharu Doi, Keiji Numata*. Biosynthesis and Characterization of Polyhydroxyalkanoate Containing 5-Hydroxyvalerate (5HV) Units: Effects of 5HV Units on Biodegradability, Cytotoxicity, Mechanical and Thermal Properties. Polymer Degradation and Stability, Volume 98, Issue 1, pp331-338, 2013.
  113. Sachiko Kaihara Nitta, Keiji Numata*. Biopolymer-based Nanoparticles for Drug/Gene Delivery and Tissue Engineering. International Journal of Molecular Sciences, Special Issue "Bioactive Nanoparticles 2012", Volume 14, Issue 1, pp1629-1654, 2013.
    Open access
  114. Keiji Numata*. "Silk Hydrogels for Tissue Engineering and Dual Drug Delivery." Silk Biomaterials in Tissue Engineering and Regenerative Medicine, Edited by S.C.Kundu, 2013.
  115. Keiji Numata*, Yoko Motoda, Satoru Watanabe, Takanori Kigawa and Yoshiharu Doi. Active Intermediates of Polyhydroxyalkanoate Synthase from Aeromonas caviae in Polymerization Reaction. Biomacromolecules, Volume13, Issue 11, pp3450–3455, 2012.
  116. Keiji Numata*, Shoya Yamazaki, Naofumi Naga. Biocompatible and Biodegradable Dual-Drug Release System Based on Silk Hydrogel Containing Silk Nanoparticles. Biomacromolecules, Volume 13, Issue 5, pp1383-9, 2012 (Most read paper between April and June 2012).
  117. Peter James Baker, Keiji Numata*, Chemo-Enzymatic Synthesis of Poly(L-Alanine) in Aqueous Environment. Biomacromolecules, Volume 13, Issue 4, pp947-51, 2012.
    Open access
  118. Keiji Numata*, Yoshiharu Doi. Biosynthesis of Polyhydroxyalkanoate by Novel Facultative Anaerobe Vibrio sp. under Marine Conditions. Marine Biotechnology, Volume 14, Issue 3, pp323-331, 2012.
  119. Peter J Baker, Keiji Numata*. "Polymerization of Peptide Polymers for Biomaterial Applications" Polymer Science, ISBN 979-953-307-969-0. 2012.
    Open access
  120. Keiji Numata*, Takuya Katashima, Takamasa Sakai. The State of Water, Molecular Structure and Cytotoxicity of Silk Hydrogels. Biomacromolecules, Volume 12, Issue 6, pp2137-44,2011.
  121. Keiji Numata*, Yoshiharu Doi. Dual Biosyntheses of Poly[(R)-3-hydroxybutyric acid] and Silk Protein to Fabricate Bio-functional Bioplastic. Polymer Journal, Volume 43, pp642-647, 2011 (Selected as a Featured Article, Top3 Downloaded Article in July 2011)

Publications (Previous works)

  1. Keiji Numata, Aneta J Mieszawska, Laura A. Kvenvold, David L Kaplan*. Silk-Based Nano-Complexes with Tumor Homing Peptides for Tumor-Specific Gene Delivery. Macromolecular Bioscience Volume 12, Issue 1, pp75-82, 2012.
  2. Keiji Numata, Michaela R Reagan, Robert H Goldstein, Michael Rosenblatt, David L Kaplan*. Spider Silk-Based Gene Carriers for Tumor Cell-Specific Delivery. Bioconjugate Chemistry, Volume 22, Issue 8, pp1605-1610,2011.
  3. Vikas Yadav, Bruce Panilaitis, Hai Shi, Keiji Numata, Kyongbum Lee, David L Kaplan*. N-Acetylglucosamine 6-Phosphate Deacetylase (nagA) Is Required for N-acetyl Glucosamine Assimilation in Gluconacetobacter xylinus. PLoS ONE 6(6): e18099, 2011.
  4. Sílvia Gomes, Keiji Numata, Isabel B. Leonor, João F. Mano, Rui L. Reis, David L. Kaplan*. AFM Study of Morphology and Mechanical Properties of A Chimeric Spider Silk and Bone Sialoprotein Protein for Bone Regeneration, Biomacromolecules, Volume 12, Issue 5, pp1675-1685,2011.
  5. Keiji Numata, and David L. Kaplan*. Differences in Cytotoxicity of β-Sheet Peptides Originated from Silk and Amyloid β. Macromolecular Bioscience Volume 11, Issue 1, pp60-64,2011.
  6. Keiji Numata, David L Kaplan*. "Chapter 20: Biologically-Derived Scaffolds", Advanced Wound Repair Therapies, Edited by David Farrar and Bryan Greener, pp524-551, Woodhead Publishing, 2011.
  7. Sejuti Sengupta, Sang-Hyug Park, Eun Seok Gil, Atur Patel, Keiji Numata, Chia-Li Lu, David L. Kaplan*. Quantifying Osteogenic Cell Degradation of Silk Biomaterials. Biomacromolecules Volume 11, Issue 12, pp3592-3599, 2010.
  8. Keiji Numata, David L Kaplan*. Silk-Based Gene Delivery Vehicles to Contain Membrane-Destabilizing Peptides: Control of Transfection by Their Secondary Structure. Biomacromolecules Volume 11, Issue 11, pp3189-3195, 2010.
  9. Keiji Numata, Juliana Hamasaki, Balajikarthick Subramanian, David L Kaplan*. Gene Delivery Mediated by Recombinant Silk Proteins Containing Cationic and Cell Binding Motifs. Journal of Controlled Release Volume 146, Issue 1, pp136-143, 2010.
  10. Nina Tandon, Anna Marsano, Robert Maidhof, Keiji Numata, Chrystina Montouri-Sorrentino, Christopher Cannizzaro, Joel Voldman, Gordana Vunjak-Novakovic*. Surface-Patterned Electrode Bioreactor for Electrical Stimulation. Lab on a Chip Volume 10, Issue 6, pp692-700, 2010.
  11. Keiji Numata, David L Kaplan*. Mechanisms of Enzymatic Degradation of Amyloid Beta Microfibrils Generating Nanofilaments and Nanospheres Related to Cytotoxicity. Biochemistry Volume 49, Issue 15, pp3254-3260, 2010.
  12. Rangam Rajkhowa, Eun Seok Gil, Jonathan Kluge, Keiji Numata, Lijing Wang, Xungai Wang, David L Kaplan*. Reinforcing Silk Scaffolds with Silk Particles. Macromolecular Bioscience Volume 10, Issue 6, pp599-611, 2010.
  13. Keiji Numata, Peggy Cebe, David Kaplan*. Mechanism of Enzymatic Degradation of Beta Sheet Crystals. Biomaterials Volume31, Issue 10, pp2926-2933, 2010.
  14. Guokui Qin, Shaul Lapidot, Keiji Numata, Xiao Hu, Sigal Meirovitch, Mara Dekel, Itai Podoler, Oded Shoseyov, David L. Kaplan*. Expression, Cross-Linking and Characterization of Recombinant Chitin Binding Resilin. Biomacromolecules Volume 10, Issue 12, pp3227-3234, 2010.
  15. Keiji Numata, David L Kaplan*. Silk-Based Delivery Systems of Bioactive Molecules. Advanced Drug Delivery Reviews Volume 62, Issue 15, pp1497-1508, 2010.
  16. Keiji Numata, Balajikarthick Subramanian, Heather Currie, David Kaplan*. Bioengineered Silk Protein-Based Gene Delivery Systems. Biomaterials Volume 30, Issue 29, pp5775-5784, 2009.
  17. Keiji Numata, Hideki Abe, Tadahisa Iwata*. Biodegradability of Poly(hydroxyalkanoate) Materials. Materials Volume 2, Issue 3, pp1104-1126, 2009.
    Open access
  18. Keiji Numata, Anna Finne-Wistrand, Ann-Christine Albertsson, Yoshiharu Doi, Hideki Abe*. Enzymatic Degradation of Monolayer for Poly(lactide) Revealed by Real-Time Atomic Force Microscopy: Effects of Stereochemistry, Molecular Weight and Molecular Branches on Hydrolysis Rate. Biomacromolecules Volume 9, Issue 8, pp2180-2185,2008.
  19. Keiji Numata*, Hideki Abe, Yoshiharu Doi. Enzymatic Processes for Biodegradation of Poly(hydroxyalkanoate)s Crystals. Canadian Journal of Chemistry (Special Issue: Dedicated to Professor R. Marchessault) Volume 86, Issue 6, pp471-483,2008.
  20. Keiji Numata, Rajiv K. Srivastava, Anna Finne-Wistrand, Ann-Christine Albertsson*, Yoshiharu Doi, Hideki Abe*. Branched Poly(lactide) Synthesized by Enzymatic Polymerization: Effects of Molecular Branches and Stereochemistry on Enzymatic Degradation and Alkaline Hydrolysis. Biomacromolecules Volume 8, Issue 10, pp3115-3125,2007.
  21. Keiji Numata, Koichi Yamashita, Masahiro Fujita, Takeharu Tsuge, Ken-ichi Kasuya, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*.Adsorption and Hydrolysis Reactions of Poly(hydroxybutyric acid) Depolymerases Secreted from Ralstonia pickettii T1 and Penicillium funiculosum onto Poly[(R)-3-hydroxybutyric acid]. Biomacromolecules Volume 8, Issue 7, pp2276-2281,2007.
  22. Keiji Numata, Shun Sato, Masahiro Fujita, Takeharu Tsuge, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*.Adsorption Effects of Poly(hydroxybutyric acid) Depolymerase on Chain-Folding Surface of Polyester Single Crystals Revealed by Mutant Enzyme and Frictional Force Microscopy.  Polymer Degradation and Stability Volume 92, Issue 1, pp176-183,2007.
  23. Keiji Numata, Yoshihiro Kikkawa, Takeharu Tsuge, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*.Adsorption of Biopolyester Depolymerase on Silicon Wafer and Poly[(R)-3-hydroxybutyric acid] Single Crystal Revealed by Real-Time Atomic Force Microscopy.  Macromolecular Bioscience Volume 6, Issue 1, pp41-50,2006.
  24. Koichi Yamashita*, Miwa Yamada, Keiji Numata, Seiichi Taguchi.Nonspecific Hydrophobic Interactions of a Repressor Protein, PhaR, with Poly[(R)-3-hydroxybutyrate] Film Studied by Quartz Crystal Microbalance. Biomacromolecules Volume 7, Issue 8, pp2449-2454,2006.
  25. Keiji Numata, Yoshihiro Kikkawa, Takeharu Tsuge, Tadahisa Iwata, Yoshiharu Doi, Hideki Abe*. Enzymatic Degradation Processes of Poly[(R)-3-hydroxybutyric acid-co-(R)-3-hydroxyvaleric acid] Single Crystals Revealed by Atomic Force Microscopy: Effects of Molecular Weight and Second-Monomer Composition. Biomacromolecules Volume 6, Issue 4, pp2008-2016,2005.
  26. Takeharu Tsuge, Kohtaroh Yano, Shin-ichi Imazu, Keiji Numata, Yoshihiro Kikkawa, Hideki Abe, Seiichi Taguchi, Yoshiharu Doi*. Biosynthesis of Polyhydroxyalkanoate (PHA) Copolymers from Fructose Using Wild-Type and Laboratory-Evolved PHA Synthases. Macromolecular Bioscience Volume 5, Issue 2, pp112-117, 2005.
  27. Keiji Numata, Takuya Hirota, Yoshihiro Kikkawa, Takeharu Tsuge, Tadahisa Iwata, Hideki Abe, Yoshiharu Doi*. Enzymatic Degradation Processes of Lamellar Crystals in Thin Films for Poly[(R)-3-hydroxybutyric acid] and Its Copolymers Revealed by Real-Time Atomic Force Microscopy. Biomacromolecules Volume 5, Issue 6, pp2186-2194,2004.
  28. Yoshihiro Kikkawa, Takuya Hirota, Keiji Numata, Takeharu Tsuge, Hideki Abe, Tadahisa Iwata, Yoshiharu Doi*. In-situ Atomic Force Microscopy Observation of Enzymatic Degradation in Poly(hydroxyalkanoic acid) Thin Films: Normal and Constrained Conditions. Macromolecular Bioscience Volume 4, Issue 3, pp276-285, 2004.


  1. Takeshi Yoshizumi, Keiji Numata, Development of transplastomic spectinomycin-sensitive Arabidopsis using a fusion peptide. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 54, S93-S93, 2018.
  2. Mitsuhiro Kimura, Takeshi Yoshizumi, Keiji Numata. A Centrifugation-Assisted Peptide-mediated plant Transformation (CAPT) method for various high-throughput analyses: its protocol and efficiency. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 54, S93-S93, 2018.
  3. Keiko Midorikawa, Yutaka Kodama, Keiji Numata. Investigation on Invasion Pathway in Intact Plants of Peptide-DNA complex for Gene Delivery. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 54, S92-S92.
  4. Kosuke Tsuchiya*, Keiji Numata*. Protease-catalyzed Polymerization of Unnatural Amino Acids. Meeting abstract 267, Volume 254, 254th National Meeting and Exposition of the American-Chemical-Society 2017.
  5. Baker, Peter J.; Kobayashi, Soma; Numata, Keiji, Chemoenzymatic Synthesis of Polypeptide- Based Functional Materials, Abstracts, 38th Northeast Regional Meeting of the American Chemical Society, Rochester, NY, United States, Sept. 30-Oct. 3. Pages NERM-203, 2012.
  6. Keiji Numata, Chemoenzymatic Syntheses of Poly(L-alanine) to Develop Spider Silk Mimics, Polymer Preprints (American Chemical Society, Division of Polymer Chemistry), Volume 53, Issue 2, pp336-337, 2012.


  1. 小田原真樹、沼田圭司「細胞膜透過性ペプチドを用いた大腸菌細胞内への巨大DNA導入法」、バイオインダストリー協会機関誌 バイオとインダストリー(B&I)、第77巻6号(2019年11月10日発行).
  2. 沼田圭司、増永啓康 「放射光を利用したシルクの構造解析」、放射光学会誌「放射光」、第32巻3号(2019).
  3. 沼田圭司. 「バイオイメージングによるオルガネラ改変機構の解明とアグリバイオへの展望」、アグリバイオ、2019年1月号.
  4. 沼田圭司博士に聞く 「生物由来の素材から革新的な材料をつくる」、現代化学、2018年12月号
  5. 土屋康佑、沼田圭司、「プロテアーゼを利用した構造タンパク質モチーフの化学合成」、化学と生物、農芸化学会、2018年12月号.
  6. 土屋康佑、沼田圭司、「化学酵素重合を利用した機能性ポリペプチドの合成」、化学工業、2018年12月号.
  7. 土屋康佑、沼田圭司、「化学酵素重合法による高機能タンパク質素材の合成」、バイオサイエンスとインダストリー、バイオインダストリー協会、2018年9月号.
  8. 沼田圭司. 化学掲示板「クモの糸 合成の手がかり 理研「体内では液体状」を再現」、化学、2018年8月号.
  9. 沼田圭司. 「アミノ酸から構成される高分子材料の創成」、アグリバイオ、2018年8月号.
  10. 沼田圭司、土屋康佑、児玉豊. 「オルガネラ反応クラスターが切り拓くバイオマス社会」、植物化学調節学会、植物の生長調節、53巻1号 pp80-83, 2018年.
  11. 土屋康佑、沼田圭司、「クモ糸の分子構造を模倣する―酵素を利用した天然タンパク質モチーフの化学合成」、FRAGRANCE JOURNAL、2018年6月号.
  12. 沼田圭司. 「シルクに基づいた高分子材料の開発」、生体適合性高分子材料の開発、月刊「機能材料」2018年5月号.
  13. 土屋康佑、沼田圭司「化学酵素重合による天然を模倣したポリペプチド材料の合成」、化学と工業、日本化学会、2017年70巻6月号.
  14. 沼田圭司. 「ペプチド法:人工制限酵素をはじめとしたタンパク質の導入技術として」、植物化学調節学会、植物の生長調節2017年5月、Vol52、No1.
  15. 沼田圭司. 「リグニンおよびその誘導体を利用したバイオマスプラスチックの微生物合成」、触媒学会 触媒、2016年58巻2号.
  16. 沼田圭司. 「深海微生物によるバイオプラスチック生産」、現代科学、2016年1月号.
  17. 沼田圭司. 「構造タンパク質の基礎物性を理解する −構造材料への利用に向けて−」、高分子学会、高分子、2015年12月号.
  18. 沼田圭司. 「植物ミトコンドリアへの選択的遺伝子導入方法の開発」、バイオサイエンスとインダストリー、バイオインダストリー協会、73巻4号、2015.
  19. 沼田圭司. 「ポリペプチドの化学酵素重合および機能材料への応用」、高分子学会超分子研究会、超分子アニュアルレビューNo.35、2015.
  20. 沼田圭司. 「植物を利用したバイオプラスチック合成」、植物化学調節学会、植物の生長調節2014年12月、Vol49、No2.
  21. 沼田圭司.  Hot Topics「A new peptide synthesis via chemo-enzymatic reactions」高分子、2014年9月号.
  22. 沼田圭司.  Hot Topics「Biosynthesis of poly- and oligo-hydroxyalkanoate in marine bacteria.」高分子、2014年8月号.
  23. 沼田圭司、富澤哲. 「リグニン構成成分を原料としたバイオプラスチックの微生物合成」、紙パルプ技術タイムス2014年8月号
  24. 沼田圭司. 「酵素触媒によるバイオマスからのバイオポリマー合成とその展望」、日本油化学会誌 オレオサイエンス、2014年第14巻第3号.
  25. 沼田圭司. 「二酸化炭素からバイオプラスチックを合成〜植物による化成品生産を可能にする植物遺伝子導入技術〜」月刊プラスチック、2014年3号
  26. 沼田圭司. 「植物による物質生産を目指した新規植物遺伝子導入技術ペプチド法の開発」 化学経済, 2013年11月号.
  27. 沼田圭司.「生体触媒によるバイオ物質生産技術の創成」フジサンケイビジネスアイ、2013年5月16日.

Invited lecture & seminar

  1. Keiji Numata, TBD. Symposium “Bioinspired synthetic polymers: synthesis, self-assembly and application” Pacifichem International Chemical Congress, Honolulu, Hawaii, December 15-20, 2020(予定).
  2. 沼田圭司、アミノ酸からなる高分子の機能性と構造性. ポリマー材料フォーラム、2020年11月26-27日(予定).
  3. Keiji Numata, TBD. The 10th China International Silk Conference, Suzhou, China, September 18-20, 2020(予定).
  4. 沼田圭司、ペプチドを利用した植物オルガネラ改変. 日本植物細胞分子生物学会、2020年9月11-12日(予定).
  5. Keiji Numata, TBD. 2020 IUPAC-World Polymer Congress (MACRO2020), Jeju Island, South Korea July 5-9, 2020(予定).
  6. Keiji Numata, TBD. International Symposium on Polymer Physics (PP2020), Chongqing, Chiana, June 14-18, 2020(延期).
  7. Keiji Numata, Transformation platform and biopolymer productivity of photosynthetic bacteria and algae. The 7th Conference of the International Society for Applied Phycology, Makuhari, Japan, April 19-24, 2020(延期).
  8. Keiji Numata, Polypeptides as functional nanomaterials toward achieving plant organellar engineering through cell walls. Renewable Molecules & Materials: Anselme Payen Award Symposium in Honor of Ann-Christine Albertsson, ACS Spring National Meeting, Philadelphia, March 23-26, 2020(中止).
  9. 沼田圭司、ポリアミノ酸、ポリペプチドの材料化技術開発. 高分子同友会、高分子学会、2020年3月18日(延期).
  10. 沼田圭司、我が国におけるバイオマスプラスチック. 廃棄物資源循環学会セミナー、一般社団法人廃棄物資源循環学会、専売ホール(東京田町)、2019年12月23日.
  11. Keiji Numata, Silk-based materials as eco-friendly material. Materials Research Meeting 2019, December 10-14, 2019, Yokohama, Japan.
  12. 沼田圭司、ペプチドを利用したオルガネラ改変. 第66 回日本放線菌学会学術講演会、2019年11月29日(予定).
  13. Keiji Numata, Biodegradability in hierarchical polymer structures. Chemical Sciences and Society Summit, London, November 11-14, 2019.
  14. 沼田圭司、試験管の中でクモ糸を自由に作る、CSJ化学フェスタ2019、日本化学会、2019年10月17日.
  15. Keiji Numata, Rationally-designed polypeptides based on natural structural proteins. The Seventh RIKEN–NCTU (台湾国立交通大学) Symposium on Physical and Chemical Science, Taiwan, Ocotober 4-5, 2019.
  16. 沼田圭司、アミノ酸を基本骨格とする高分子の化学酵素重合および繊維の階層構造形成に関する研究(受賞講演)、高分子討論会、2019年9月26日.
  17. 沼田圭司、ペプチド法:融合ペプチドを利用した植物改変技術の開発(受賞講演)、日本植物細胞分子生物学会、2019年9月7日.
  18. Keiji Numata, Rationally-designed polypeptides based on structural proteins. Future of Biomacromolecules at a Crossroads of Polymer Science & Biology, ACS Fall National Meeting, San Diego, CA, August 25-29, 2019.
  19. Keiji Numata, Spider silk fibers and plant cells as soft matter. Porf Masao Doi's Lab Seminar, Beihang University, China, July 3, 2019.
  20. 沼田圭司、クモ糸の階層構造の解明とエコマテリアルとしての可能性、九州大学 先導物質化学研究所講演会、2019年7月27日.
  21. Keiji Numata, Chemoenzymatic synthesis of polypeptides based on spider silk spinning mechanism. 2019 United States-Japan Polymer Symposium, Stanford University, USA, June 18-21, 2019.
  22. Keiji Numata, Rationally-designed silk materials based on the spinning mechanism. Silk Conference 2019, Trento, Italy, June 13-16, 2019.
  23. Keiji Numata, Chemoenzymatic polymerization and photosynthetic bacterial synthesis of artificial structural protein polymers. 26th Anniversary meeting of the Bio-Environmental Polymer Society (BEPS), Clemson University, USA, June 5-7, 2019.
  24. 沼田圭司 ”バイオ”に立脚した高分子材料の創出、第14回理研「バイオものづくり」シンポジウム、理化学研究所、2019年3月8日.
  25. Keiji Numata, Polypeptides as functional nanomaterials toward achieving organellar engineering and artificial spider silk. International Conference on Functional Nanomaterials (ICFNM)-2019, Varanasi, India, February 22-25, 2019.
  26. Keiji Numata, Genetic modification of mitochondria. Hubrecht Seminar, Hubrecht Institute, Germany, February 14, 2019.
  27. Keiji Numata. Polypeptides as biological structural materials. Special MEC Seminar, California Institute of Technology, USA, January 15, 2019.
  28. 鈴木隆領、沼田圭司、放射光が示すクモ糸の分子機構と材料設計、内閣府ImPACTプログラムにおける放射光施設の活用と展開、放射光学会、九州国際会議場、2019年1月10日(予定).
  29. Keiji Numata, Spider silk-mimicking polypeptide-Mechanism in nature & chemoenzymatic synthesis-, The Society of Polymer Science-India (SPSI) Biannual Meeting, MACRO-2018, Pune, India, December 19-21, 2018.
  30. 沼田圭司、クモ糸の紡糸機構から学ぶ高分子設計、第166回東海高分子研究会講演会、名古屋大学、2018年12月15日.
  31. 沼田圭司、ペプチドを利用した植物バイオ技術、植物科学シンポジウム、東京大学、2018年12月12日.
  32. Keiji Numata, Molecular design and chemoenzymatic synthesis of structural polypeptides. The 12th SPSJ International Polymer Conference (IPC2018), Hiroshima, Japan, December 4th-7th, 2018.
  33. Keiji Numata, Polypeptide materials for agricultural and engineering applications. BASF NAO Familyday, BASF, Shanghai, November 1st, 2018.
  34. 沼田圭司、クモ糸で繋がるエンジニアリングネットワーク、第二回ものづくりワークショップ、理化学研究所 バイオサイエンスハブ、2018年10月3日.
  35. Keiji Numata, Rationally-designed polypeptides based on spider silk.日台二カ国高分子シンポジウム, 国立中正大学, Taiwan, September 30th- October 4th, 2018.
  36. 沼田圭司、クモ糸の階層構造の解明と人工的再構築. 生物工学会 シンポジウム「スマートバイオデザイン:ナノからマクロスケールまでの様々な階層レベルにおけるバイオ関連機能と構造の合理的創出を目指して」、関西大学千里山キャンパス、2018年9月7日.
  37. Keiji Numata, Chemo-enzymatic synthesis of silk and elastin-like polypeptides as renewable materials. Symposium on The Rational Design of Multifunctional Renewable-Resourced Materials, ACS National Meeting, Boston, Massachusetts, August 19-23, 2018.
  38. Keiji Numata, Enzymatic synthesis of structural protein-like polypeptides. BioEnvironmental Polymer Society 2018, Rensselaer Polytechnic Institute, Troy, New York, August 15-17, 2018.
  39. 沼田圭司、クモ糸の紡糸機構. 繊維学会夏季セミナー、北ビワコホテルグラツィエ、2018年8月9日.
  40. 沼田圭司、高分子科学を利用して植物科学の可能性を広げる. 高分子学会講演会、東京工業大学、2018年7月18日.
  41. Keiji Numata, Molecular design and biosynthesis of structural proteins based on their hierarchical structures in nature. Beihang Unversity, June 5, 2018.
  42. Keiji Numata, Rationally-designed polypeptides based on nature and polymer science. Symposium for the 50th Anniversary of Polymer Journal, Nagoya Congress Center, May 23, 2018.
  43. 沼田圭司、構造材料を志向したポリペプチドの分子設計. 第25回関西若手高分子セミナー、京都大学桂キャンパス、2018年5月9日.
  44. Keiji Numata, Rationally-designed polypeptides based on structural proteins. 111 Center Symposium, Qingdao, China, April 23-26, 2018.
  45. Keiji Numata, Polypeptide-based micelles to modify plant cells. 日本化学会第98春季年会アジア国際化学シンポジウムナノテク・材料化学ディビジョン、日本大学理工学部 船橋キャンパス、2018年3月20-23日.
  46. Keiji Numata, Potential of polypeptide-based materials: from artificial spider silk to gene delivery system, Department Lecture, Universität Bayreuth, Germany, January 18, 2018.
  47. Keiji Numata, Chemoenzymatic synthesis of structural and functional polypeptides, Department Seminar, Royal Institute of Technology (KTH), Sweden, January 15, 2018.
  48. Keiji Numata, Polypeptides as structural and functional materials, HPJ異分野融合ワークショップFrontiers in materials, sensors and devices for humanophilic innovation, 奈良先端科学技術大学院大学, November 20, 2017.
  49. Keiji Numata, Potential of polypeptide-based materials: from spider silk to gene delivery, Department seminar, Deakin University, November 7th, 2017.
  50. Keiji Numata, Potential of polypeptide-based materials: from spider silk to gene delivery, Evolution & Ecology Research Centre Seminar, University of New South Wales, November 3rd, 2017.
  51. Kenjiro Yazawa, Keiji Numata. Use of Silk as Structural Material. The 5th international conference on smart systems engineering 2017 (SmaSys2017),山形大学米沢キャンパス、2017年10月12-13日.
  52. 沼田圭司、ポリペプチドと高分子科学〜オルガネラ工学から人工クモ糸まで〜. IGERグリーン自然科学レクチャー、名古屋大学リーディング大学院グリーン自然科学国際教育研究プログラム、2017年10月5日.
  53. 沼田圭司、エコナノファイバーから構成される構造タンパク質材料の創成. 日本学術振興会 先端ナノデバイス・材料テクノロジー第151委員会研究会、2017年9月15日(予定).
  54. 矢澤健二郎、沼田圭司、人工クモ繊維・開発と評価法. 第15回SPring-8先端利用技術ワークショップ~生体システムを利用した新しい機能性材料とその起源~、公益財団法人 高輝度光科学研究センター、キャンパスプラザ京都、2017年8月24日.
  55. Kosuke Tsuchiya, Keiji Numata. Chemoenzymatic polymerization of unnatural amino acids. ACS Fall Meeting, Green Polymer Chemistry: Biobased Materials & Biocatalyst, August 20-24, 2017.
  56. 沼田圭司、ペプチドの化学酵素合成と材料設計〜オルガネラ工学からクモ糸まで〜. 若手ペプチド夏の勉強会、長崎スカイホテル、2017年8月5-7日.
  57. Keiji Numata, Chemoenzymatic synthesis of biopolymers to design artificial spider silk, Japan-South-East Asia Collaboration Hub of Bioplastics Study, JSPS-Core-to-Core Program, University of Tokyo, August 3, 2017.
  58. Mieko Higuchi-Takeuchi, Kumiko Morisaki, Keiji Numata. Polyhydroxyalkanoate production by marine purple photosynthetic bacteria. JSPS拠点シンポジウム、大阪大学、2017年7月31日-8月1日(予定).
  59. Keiji Numata, Potential of peptide-based materials: from spider silk to gene delivery. Beihang Unversity, June 22, 2017.
  60. Keiji Numata. Material design and chemo-enzymatic synthesis of structural protein polymers. The 6th International Conference on Bio-based Polymers 2017 (ICBP2017), Yuan Ze University, Taiwan, May 14-17, 2017.
  61. 沼田圭司、New insights into spider dragline spinning. 日本化学会アジア国際シンポジウム(高分子部門)慶應大学日吉キャンパス、2017年3月18日.
  62. 吉積毅、沼田圭司、ペプチドを用いたゲノム編集周辺技術の開発、第58回日本植物生理学会年会 シンポジウム 植物機能の解明を目指すゲノム編集技術、2017年3月.
  63. Keiji Numata, Chemo-enzymatic synthesis of structural and functional protein polymers. BASF agora, Kyoto Univeristy, February 24, 2017.
  64. Keiji Numata. Peptide-based transformation technologies for plant organelles. Gordon Research Conference 2017 “Chloroplast Biotechnology”, Ventura, CA, January 8-13, 2017.
  65. 沼田圭司、「ゲノム編集技術をいかに植物に利用するか」、日本農業経営大学校 特別講義、大手町サンケイプラザ 会議室、2016年12月8日.
  66. 沼田圭司、「人工シルクをいかに設計・合成するか 〜その課題と現状〜」、第2回社会に貢献する理研の工学研究シンポジウム、理化学研究所、2016年11月17日.
  67. Keiji Numata. Silk-based structural materials inspired by hierarchical structures and functions of spider silk. Beihang University Advanced Materials Series, November 2, 2016.
  68. Keiji Numata. Gene/protein delivery using peptide-based carriers to edit organellar and nuclear genomes. 2016 International Symposium on Plant Transformation Biotechnologies, Institute of Plant and Microbial Biology (IPMB), Academia Sinica, Taipei, October 17-19, 2016.
  69. 沼田圭司、ペプチドによるタンパク質導入技術を利用したゲノム編集、第80回植物学会シンポジウム「植物から菌まで〜多様な生命の謎を探り生かすGenome Editing」、沖縄コンベンションセンター、2016年9月16-19日.
  70. 沼田圭司、Spider silk-like polypeptides: Enzymatic synthesis and materials design with water molecules. 高分子学会2016年度討論会日韓セッション若手講演、神奈川大学、2016年9月14-16日.
  71. 沼田圭司、Hierarchical structures and functions of spider silk.日本蜘蛛学会大会 公開シンポジウム、東京大学柏キャンパス、2016年8月20日.
  72. 沼田圭司、構造材料を志向したポリペプチドの生合成と材料設計、関東高分子若手研究会サマーキャンプ、東京農工大館山荘、2016年8月4-6日.
  73. 沼田圭司、シルク素材のタフネスおよび熱的安定性を向上させる技術を開発-水分子が引き起こすシルクの構造変化を解明-、理研イブニングセミナー、理研東京事務所、2016年6月22日.
  74. Keiji Numata. Chemoenzymatic synthesis and material design of polypeptides. The 3rd International Symposium for Green-Innovation Polymers (GRIP2016), JAIST, March 4-5th, 2016.
  75. 土屋康佑、沼田圭司、酵素重合を利用した構造タンパク材料の開発、平成27年度日本素材物性学会研究会、秋田大学ベンチャー・ビジネス・ラボラトリー、2016年2月12日
  76. 沼田圭司、アミノ酸から構成される高分子の合成および構造材料への展開、高分子学会埼玉地区懇話会、リンテック(株)大会議場、2016年1月22日.
  77. Keiji Numata. Potential as structural materials and chemo-enzymatic synthesis of poly(amino acid), Pacifichem2015, Hawaii, U.S.A, December 15-20, 2015.
  78. 沼田圭司、植物ミトコンドリアへの選択的遺伝子導入方法の開発: 高分子および高付加価値ケミカルの生合成への展開、JBA植物バイオ研究会(八丁堀)、2015年11月24日.
  79. 沼田圭司、ペプチドを利用した新たな遺伝子導入技術「ペプチド法」の開発、かずさDNA研究所セミナー、2015年11月2日.
  80. Keiji Numata. Silk and Related Peptides as Structural Materials, 5TH MOLECULAR MATERIALS MEETING (M3) @ SINGAPORE, August 3-5, 2015.
  81. Keiji Numata. Organelle-targeted gene delivery using peptide-based carriers, The 13th International Conference of Polymers for Advanced Technologies (PAT2015), Zhejiang University (Hangzhou, China), June 25-28, 2015.
  82. Mieko Higuchi-Takeuchi & Keiji Numata. Bioplastic production by phototrophic bacteria using CO2. 7th International Conference on Green and Sustainable Chemistry (GSC-7) and 4th JACI/GSC Symposium. Tokyo, July 7, 2015.
  83. Kenjiro Yazawa & Keiji Numata. Thermal property of silk fibroin: relationship between glass transition temperatures and water molecules. 7th International Conference on Green and Sustainable Chemistry (GSC-7) and 4th JACI/GSC Symposium. Tokyo, July 7, 2015.
  84. Keiji Numata. Peptide-based gene carriers for organelle-targeted delivery system, 2015 Symposium for the promotion of applied research collaboration in Asia (SPARCA 2015), Taipei, Taiwan, February 8-11, 2015.
  85. 沼田圭司、ペプチド法による植物への遺伝子導入、植物ゲノム編集ワークショップ、岡山大学植物研、2014年11月4日.
  86. 沼田圭司、ペプチドの化学酵素合成およびバイオマス材料としての応用、第4回CSJ化学フェスタ2014、日本化学会、2014年10月15日.
  87. Keiji Numata. Structural analysis of silk fibers and importance of the beta-sheet formation. International Silk Conference, Fudan University, Shanghai, October 8th, 2014.
  88. 沼田圭司、リグニンを利用した生分解性プラスチックの開発とバイオリファイナリーへの展開、~難利用木質バイオマス徹底活用~リグニン産業最前線 2014、サイエンス&テクノロジー株式会社、2014年9月18日.
  89. 沼田圭司、ペプチドと核酸の複合体を利用した植物への遺伝子導入法の開発、新学術領域「植物細胞壁機能」セミナー、奈良先端科学技術大学院大学、2014年7月14日.
  90. 沼田圭司、Synthesis of poly- and oligo(hydroxyalkanoate)s by deep-sea bacteria, Colwellia spp., Moritella spp., and Shewanella spp.(受賞講演)、第63回高分子学会年次大会、名古屋国際会議場、2014年5月.
  91. Nicholas Thomson, Jo-Ann Chuah, Keiji Numata. Increasing the range and performance of polyhydroxyalkanoates. ABS - 1512 – ICA, (18) Hybrids including Biomass based Materials, International Union of Materials Research Society- International Conference in Asia 2013, 19-12-2013.
  92. Keiji Numata. Peptides for Green Innovations. Chinese Academy of Sciences. Invited Seminar, November 7th, 2013
  93. Jose Manuel Ageitos, Keiji Numata. Protease-mediated synthesis of peptides in aqueous solution. Chinese Academy of Sciences. Invited Seminar, November 7th, 2013
  94. 沼田圭司、酵素で高分子を合成する、バイオマスイノベーション若手の会第一回キックオフワークショップ、東京大学、2013年11月6日.
  95. 沼田圭司、バイオマスからの新規プラスチック生産戦略―高分子科学の視点で挑む「二酸化炭素の資源化」および「バイオマスコンビナートと次世代農業の融 合」―、近畿バイオインダストリー振興会議バイオマス研究会公開セミナー、大阪科学技術センター、2013年9月4日.
  96. Keiji Numata. Silk hydrogel with high strength and biocompatibility. Tissue Engineering and Regenerative Medicine International Society – Asia Pacific Chapter (TERMIS-AP) 2013 annual conference, Shanghai and Wuzhen, PR China, 25 October 2013.
  97. 沼田圭司、Biosynthesis and agricultural applications of biopolymers: Recent update on silk and bioplastics、先端生命科学研究所セミナー、慶応義塾大学、2013年7月.
  98. 沼田圭司、環境・植物工学への応用を目指したバイオポリマー設計、九州大学、2013年4月.
  99. 沼田圭司、植物による物質生産を目指した新規遺伝子導入「ペプチド法」、第19回C-Bioセミナー、宇都宮大学、2013年1月.
  100. 沼田圭司、酵素触媒を用いた新規バイオポリマー合成、植物科学シンポジウム 植物最先端研究への期待、品川、コクヨホール、2012年12月3日.
  101. 沼田圭司、バイオポリエステルおよびバイオポリアミドの生合成とその応用、エコマテリアル研究会、東京、2012年7月.
  102. 沼田圭司、バイオポリマーの生合成およびグリーンイノベーションへの利活用、播磨理研SPring-8セミナー、2012年5月.
  103. 沼田圭司、ペプチドポリマーによるソフトマテリアルデザイン、第二回新規材料創成を目指した合成生物学、横浜、2012年1月.
  104. Keiji Numata. Material design of silk polymers for hydrogel and nanoparticle. The 3rd International Conference on Biobased Polymers (ICBP2011), China, October 2011.
  105. Keiji Numata. Recent update from RIKEN Enzyme Research Team, Royal Institute of Technology, Sweden, October 2011.
  106. Keiji Numata, Yoshiharu Doi. Production and Material Design of Bio-Based Polyesters Engineering Plastic 2011, China, August 2011.
  107. Keiji Numata, Yoshiharu Doi. Bio-based and biodegradable silk protein-bacterial polyester hybrid materials. Pacifichem 2010, 854, U.S.A., December 2010.
  108. Keiji Numata, Yoshiharu Doi. Microbial production and biodegradation of polyhydroxyalkanoates. Pacifichem 2010, 847, U.S.A., December 2010.
  109. Keiji Numata. Biosyntheses of functional polymers for various applications. RIKEN Plant Science Center Retreat, Japan, October 2010.
  110. Keiji Numata, Masahiro Fujita, Yoshiharu Doi. Adsorption and hydrolysis mechanisms of PHA depolymerases. The 12th International Symposium on Biological Polyesters, 11A, Germany, October 2010.

Grants & funding

  1. 科研費助成金 挑戦的研究(萌芽) 2019-2021年 葉緑体からミトコンドリアへの細胞内遺伝子移行の検証(代表:小田原真樹)
  2. 科研費助成金 若手研究 2019-2021年 活性酸素種分解性ポリペプチドによるオルガネラ選択的バイオ医薬品デリバリー(代表:河崎陸)
  3. 科研費助成金 若手研究 2019-2021年 ゲノム編集技術を利用した汎用的プラスチド形質転換方法の開発(代表:渡邊健太)
  4. 科研費助成金 若手研究 2019-2021年 機能性ペプチドを修飾したナノミセルによる植物ミトコンドリアへの高効率な遺伝子導入(代表:宮本昂明)
  5. 科研費助成金 若手研究 2019-2020年 構造が規定された新規ポリマーベシクルの創製(代表:藤田聖矢)
  6. 科研費助成金 若手研究 2019-2020年 弾性タンパク質を模倣したペプチドエラストマーの化学酵素合成と機能評価(代表:曽川洋光)
  7. 吉田科学技術財団研究者派遣助成, Prashant Gopal Gudeangdi, 2018年度.
  8. 科研費助成金 基盤研究(C)(一般)H30-32年度 ミトコンドリアゲノム形質転換植物体の作出に向けた革新的遺伝子導入技術の開発(代表:木村光宏)
  9. 発酵研究所 平成30年度(2018年度)一般研究助成 海洋性紅色光合成細菌によるバイオポリエステル生産システムの開発(代表:樋口美栄子)
  10. 科研費助成金 挑戦的研究(萌芽)H29-H31年度 葉緑体への核酸運搬ペプチドを用いた巨大遺伝子導入法の開発(代表:吉積毅)
  11. 理化学研究所 産業連携促進に関する課題「非天然アミノ酸構造を持つマルチブロックポリペプチド材料の合成」(代表 土屋康佑)、平成29年度.
  12. 理化学研究所 エンジニアリング・ネットワーク公募型(代表 沼田圭司)、平成29年度 & 平成30年度.
  13. 科研費助成金 若手研究(B) H29-30年度 研究課題名:酵素触媒フラグメント合成を用いた高強度バイオポリアミドの開発(代表:土屋康佑)
  14. JSPS拠点形成事業 日本-東南アジアバイオプラスチック共同研究拠点(代表 宇山浩、分担参画 沼田圭司)、平成29年4月〜33年3月.
  15. 戦略的創造研究推進事業・総括実施型研究(ERATO) 沼田オルガネラ反応クラスタープロジェクト(代表 沼田圭司)、平成28年度10月〜平成33年度3月.
  16. 革新的研究開発推進プログラム(ImPACT)豊かで安全な社会と新しいバイオものづくりを実現する 人工細胞リアクタ(PM 野地博行)、プロジェクト:核酸運搬ペプチドを用いた微生物へのゲノムサイズDNA送達技術開発(吉積毅)、平成28年〜30年度.
  17. 科研費助成金 基盤研究(C) H28-30年度 研究課題名:光合成細菌を利用した二酸化炭素と窒素固定反応によるPHA生産(代表:樋口美栄子)
  18. 革新的研究開発推進プログラム(ImPACT)セレンディピティの計画的創出による新価値創造(PM 合田圭介)、プロジェクト8チーム5:バイオ高分子を高効率に生産する光合成細菌の開発(代表 沼田圭司)、平成28年度〜30年度.
  19. 松籟科学技術振興財団 研究助成 H28.3-30年3月 研究課題名:植物を宿主とした有用物質大量生産に向けた代謝経路設計法の開発(代表:吉積毅)
  20. 革新的研究開発推進プログラム(ImPACT)超高機能構造タンパク質による素材産業革命(PM 鈴木隆領)、プロジェクト:天然高機能タンパク質素材の網羅的解析と高機能発現メカニズムの解明(プロジェクトリーダー 沼田圭司)、平成26年〜30年.
  21. 戦略イノベーション創造プログラム(SIP)(次世代農林水産業創造技術)、ゲノム編集技術と開花促進技術の普及と高度化(PD 西尾健)、平成26年〜30年、一過性モジュールによる外来遺伝子を残さない植物ゲノム編集技術の開発(代表 沼田圭司).
  22. National Science Foundation 2014年度East Asia and Pacific Summer Institutes (EAPSI) プログラムH26 JSPSサマープログラム 調査研究費(代表:沼田圭司).
  23. (継続)NEDO 平成23年度 先導的産業技術創出事業(若手研究グラント)プロジェクトID:11B12007d選択的オルガネラ形質転換法の開発によるバイオ物質の大量生産(H27まで継続)(代表:沼田圭司).
  24. 科研費助成金 若手研究(B) 課題番号:25810110 H25-27年度 研究課題名:植物細胞壁を模倣したシルク-ペクチンハイドロゲルの創成とサンゴ再生基盤への利用(代表:沼田圭司)
  25. APEX DELIVERING EXCELLENCE GRANT 2012 (Malaysia), (代表 Kumar Sudesh、分担 Keiji Numata) Development of New Bio-Based Polymeric Materials Using Modified PHA Synthases for a Sustainable Tomorrow.  (2012年11月〜2014年8月).
  26. 理化学研究所 理事長ファンド若手研究会(代表 持田恵一、分担 沼田圭司), 2012-2013年度.
  27. NEDO 平成23年度 先導的産業技術創出事業(若手研究グラント)プロジェクトID:11B12007d選択的オルガネラ形質転換法の開発によるバイオ物質の大量生産(H23-H25)(代表:沼田圭司)
  28. JST先端的低炭素化技術開発事業(ALCA)FS H23年度 課題番号J2011401、植物でのPHA生産、PHAの生産量計測、植物の代謝シュミレーション(代表 松井南、分担 沼田圭司)
  29. 理化学研究所 理事長ファンド若手研究会(代表 大谷美沙都、分担 沼田圭司), 2011-2012年度.
  30. 科研費助成金 若手研究(B) 課題番号:23750264 H23-24年度 研究課題名:乳癌細胞をターゲットとした複数の機能性ペプチドから成る遺伝子ベクターの開発(沼田圭司)
  31. 住友財団 環境研究助成 プラヌラ幼生および微細藻類の人工共生系の構築を目的としたバイオポリマー材料の開発(沼田圭司),理化学研究所,2011-2012年.
  32. 理化学研究所 理事長ファンド若手研究会、代表:沼田圭司、2010-2011年度.
  33. JSPS Grant for 59th Meeting of Nobel Laureates in Lindau dedicated to Chemistry, June 28th-July 3rd 2009.
  34. 科学研究費補助金(海外特別研究員奨励費)2008-2009年度、遺伝子治療による骨再生を目的とした低炎症性および生分解性インプラントの創製、代表:沼田圭司
  35. 科学研究費補助金(特別研究員奨励費)2006-2007年度、生分解性ポリエステルの結晶および鎖における酵素分解機構の解明、代表:沼田圭司、研究課題番号:06J05679
  • [その他]  企業との共同研究費

Members’ Awards

  1. Chonprakun Thagun, 理化学研究所 桜舞賞 理研研究奨励賞, 2020年3月10日.
  2. Chonprakun Thagun, RIKEN Center for Sustainable Resource Science Presentation Award,  November 6, 2019.
  3. Nur Alia Oktaviani, 理化学研究所 桜舞賞 理研研究奨励賞, 2019年3月12日.
  4. 片島拓也、高分子学会 エコマテリアル研究会 ポスター賞、2019年3月1日
  5. Prashant Gopal Gudeangdi, 2nd-Prize in Oral Presentation. International Conference on Functional Nanomaterials (ICFNM)-2019, Varanasi, India, February 22-25, 2019.
  6. Nur Alia Oktaviani, Grant for traveling to the International Conference on Magnetic Resonance in Biological Systems (ICMRBS2018), August 2018.
  7. 土屋康佑 平成30年度 繊維学会年次大会 優秀口頭発表賞、2018年6月.
  8. 土屋康佑 RIKEN CSRS Incentive Award, “Development of artificial structural protein materials by chemoenzymatic polymerization” April 2018.
  9. Nur Alia Oktaviani Young Investigator Award, “Molecular conformation and dynamics of soluble precursor of recombinant repetitive domain of spider silk at different pH” XXVIIth International Conference on Magnetic Resonance in Biological Systems (ICMRBS2016), August 2016.
  10. Jo-Ann Chuah RIKEN CSRS Incentive Award, “Peptide-derived gene carrier for mitochondrial-targeted delivery: from plants to mammalian cells” April 2015.
  11. Yoke-Ming Wong Student Poster Presentation Award, “Optimization of poly(N-isopropylacrylamide) microgels exhibit enzyme-like amidase activity” Symposium for the promotion of applied research collaboration in Asia (SPARCA 2015), Taipei, Taiwan, February 10, 2015.
  12. Jose Ageitos RIKEN BMEP Retreat Best Poster Award, “Proteinase K mediated synthesis of peptides” December 2013.
  13. Satoshi Tomizawa RIKEN BMEP Retreat Best Poster Award, “Bacterial production of polyhydroxyalkanoate from aromatic carboxylic acid or sugar as a carbon source” December 2013.
  14. Jo-Ann Chuah RIKEN BMEP Retreat Best Poster Award, “Transient Transformation of Plant Mitochondria using Mitochondria Targeting and Cell Penetrating Peptides” December 2013.
  15. Jo-Ann Chuah Best Ph.D. Thesis Award, Universiti Sains Malaysia, September 2012.